Effectiveness of liposomes to transfect livestock fibroblasts
Authors: Raniere R. Oliveira, Daniela M. de Carvalho, Sharon Lisauskas, Eduardo Mello, Giovanni R. Vianna, Margot A.N. Dode, Rodolfo Rumpf, Francisco J.L. AragÃ?£o and ElÃ?Âbio L. Rech
The development of an efficient transfection system in livestock cells is an important step towards investigating gene transfer and the functioning and production of transgenic animals. Important factors involved in cationic liposome mediated gene transfer were evaluated through in vitro transfection of bovine, caprine and ovi.. Read More»
- Genet. Mol. Res. 4(2):
Meta-analysis of differentially expressed genes in ankylosing spondylitis
Authors: Y.H. Lee and G.G. Song
The purpose of this study was to identify differentially expressed (DE) genes and biological processes associated with changes in gene expression in ankylosing spondylitis (AS). We performed a metaanalysis using the integrative meta-analysis of expression data program on publicly available microarray AS Gene Expression Omnibus (GEO) datasets. We performed.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.6
- DOI:
- 10.4238/2015.May.18.6
Pharmacology of a traditional Chinese herb, Shuangren-Anshen capsule, in a hemorrhage mouse model by using an orthogonal array design
Authors: X.X. Liu, A. Agudamu, X. Zhang, Y. Wei, X. Song and Y.L. Li
Shuangren-Anshen capsule (SAC) is a traditional Chinese herb that was improved in our laboratory. An orthogonal experiment [L9(3)4] was used to optimize the extraction conditions. In vivo, a hemorrhage mouse model was established and the hemoglobin contents of normal control, model control, and treated mice were measured. Additionally, the sedative and hy.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038806
- DOI:
- 10.4238/gmr.15038806
Association between the interleukin 4 gene -590C>T promoter polymorphism and asthma in Xinjiang Uighur children
Authors: J.H. Zhang, G.H. Zhou, T.T. Wei and Z.S. Chang
We investigated the association between the interleukin 4 gene (IL-4) -590C>T polymorphism and forced expiratory volume in one second (FEV1) values, immunoglobulin E (IgE) levels, and susceptibility to asthma in Uighur children. IL-4 -590C>T frequencies were analyzed in 38 bronchial asthmatic patients and 35 non-asthmatic controls. Polymerase chain .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038363
- DOI:
- 10.4238/gmr.15038363
Cyclin D1 G870A gene polymorphism and risk of leukemia and hepatocellular carcinoma: a meta-analysis
Authors: Y. Zhao,H.R. He, M.Y. Wang, X.D. Ren, L. Zhang, Y.L. Dongand J. Lu
Cyclin D1 (CCND1) is a key protein involved in cellcycle regulation, and the CCND1 G870A polymorphism is associated with many types of malignancy. Studies examining the associations between this G870A polymorphism and susceptibility to leukemia and hepatocellular carcinoma (HCC) have shown inconsistent results. Therefore, we conducted a meta-analysis to c.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.7
- DOI:
- 10.4238/2015.May.18.7
Transgene elimination in genetically modified dry bean and soybean lines
Authors: Eduardo Romano, Alexandre Soares, Karina Proite, Suzana Neiva, MaÃ?Âra Grossi, Josias C. Faria, ElÃ?Âbio L. Rech and Francisco J.L. AragÃ?£o
Transgene elimination is a poorly studied phenomenon in plants. We made genetic and molecular studies of a transgenic dry bean line immune to bean golden mosaic geminivirus and a soybean line. In both lines, the transgenes were stable during the vegetative phase but were eliminated during meiosis. Due to its potential biotechn.. Read More»
- Genet. Mol. Res. 4(2):
Catheter-directed thrombolysis in the treatment of acute deep venous thrombosis: a meta-analysis
Authors: J.J. Zheng, Z.H. Zhang, Z. Shan, W.J. Wang, X.X. Li, S.M. Wang, Y.-X. Li and G.-S. Cheng
We performed a meta-analysis for systematic evaluation of the status quo of catheter thrombolysis for the treatment of acute lower limb deep vein thrombosis in China. We searched the China Biomedical bibliographic database (CBM), China National Knowledge Infrastructure (CNKI), Weipu full-text electronic journals, Wanfang full-.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.1
- DOI:
- 10.4238/2014.July.24.1
Effects of changes in serum endostatin and fibroblast growth factor 19 on the chemotherapeutic sensitivity in acute myeloid leukemia patients
Authors: Y.Z. Su, C.B. Wang, Y. Zhou and N.T. Sun
The present study aimed to explore the changes in serum endostatin and fibroblast growth factor 19 (FGF-19) in acute myeloid leukemia patients, and to determine their effects on chemotherapeutic sensitivity. Sixty acute myeloid leukemia patients and 30 healthy controls were included in the study. Patient serum endostatin and FGF-19 levels were measured on.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.8
- DOI:
- 10.4238/2015.May.18.8
Polymorphic microsatellite loci for the crimson snapper (Lutjanus erythropterus)
Authors: L. Liu, L. Lin, C.H. Li, S.N. Xu, Y. Liu and Y.B. Zhou
We isolated and characterized 22 polymorphic microsatellite loci in Lutjanus erythropterus using a (GT)13-enriched genomic library. We found between 2 and 8 alleles per locus, with a mean of 4.85. The observed and expected heterozygosities ranged from 0.065 to 0.867 and from 0.085 to 0.832, respectively, with means of.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.2
- DOI:
- 10.4238/2014.July.24.2
Abnormal expression of PTEN and PIK3CA in pemetrexed-resistant human pancreatic cancer cell line Patu8988
Authors: X. Shi, H.T. Gu, S.B. Lin, Y. Zhang, J. Yang and C.J. Qian
The aim of this study was to investigate the expression of PTEN and PIK3CA in the pemetrexed-resistant human pancreatic cancer cell line Patu8988, and to evaluate their effects on the biological behavior of pancreatic cancer cells. PTEN and PIK3CA gene and protein expressions were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and we.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036991
- DOI:
- 10.4238/gmr.15036991