Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences
Authors: B.C. Moon, W.J. Kim, Y. Ji, Y.M. Lee, Y.M. Kang and G. Choi
Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017064
- DOI:
- 10.4238/gmr.15017064
Karyotype of Rhodnius montenegrensis(Hemiptera, Triatominae)
Authors: K.C.C. Alevi, A. Ravazi, V.J. Mendon�§a, J.A. Rosa and M.T.V. Azeredo-Oliveira
The Triatominae subfamily comprises 6 tribes. The tribe Rhodniini comprises 2 genera and 22 nominal species. Rhodnius montenegrensis (Hemiptera, Triatominae) was recently described as evolutionarily related to R. robustus. Therefore, in order to contribute to karyosystematic study of the tribe Rhodniini, this report describes the number of chromosomes and.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.5
- DOI:
- 10.4238/2015.January.16.5
Genetic study of skin thickness and its association with postweaning growth in Nellore cattle: estimation of the genetic parameters
Authors: A.M. Maiorano1, M.C.S. Oliveira2, J.N.S.G. Cyrillo3, L.G. Albuquerque1,R.A. Curi4 and J.A.IIV. Silva4
The objective of the present study was to estimate genetic parameters for skin thickness (ST) and postweaning weight gain (PWG550) in Nellore cattle. Records were obtained from 152,392 Nellore animals born between 2001 and 2011. ST was measured in the posterior region of the animal’s scapula with a millimeter caliper. The animals were assigned to di.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017124
- DOI:
- 10.4238/gmr.15017124
Evaluation of methods of DNA extraction from Staphylococcus aureus in milk for use in real-time PCR
Authors: A.G. Dibbern, B.G. Botaro, M.P. Viziack, L.F.P. Silva and M.V. Santos
The aim of this study was to evaluate the repeatability and performance of 4 methods of extracting DNA from Staphylococcus aureus (SAU) and the gene encoding bovine mitochondrial cytochrome B (BMCB) in milk samples from cows with subclinical mastitis for use in amplification by real-time polymerase chain reaction. Two milk samples were obtained from cows .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.6
- DOI:
- 10.4238/2015.January.16.6
Isolation and characterization of novel polymorphic microsatellite loci in Perinereis aibuhitensis
Authors: Y. Liu, D. Yu, Q. Wang, H. Liu, S. Guan and M. Liu
Perinereis aibuhitensis is a commercially and ecologically important intertidal worm. In this study, eight polymorphic microsatellite loci were isolated in this species for the first time, and a wild population was used to estimate the properties of these loci. The number of alleles per locus ranged from 6 to 20, and the observed and expected heterozygosi.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017144
- DOI:
- 10.4238/gmr.15017144
Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle
Authors: M.M. Dias, F.R.P. Souza, L. Takada, F.L.B. Feitosa, R.B. Costa,I.D.P.S. Diaz, D.F. Cardoso, R.L. Tonussi, F. Baldi,L.G. Albuquerque and H.N. Oliveira
The objective of this study was to identify associations between known polymorphisms in genes related to adipose tissue and sexual precocity in Nellore cattle. A total of 1689 precocious and non-precocious heifers belonging to farms participating in Conexão Delta G breeding program were studied. SNPs from the Illumina High-Density Bovine SNP BeadCh.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.7
- DOI:
- 10.4238/2015.January.16.7
Indirect selection of industrial tomato genotypes that are resistant to spider mites (Tetranychus urticae)
Authors: J.E. Baier, J.T.V. Resende, M.V. Faria, K. Schwarz and L. Meert
Acyl sugars present in the tomato Solanum lycopersicum ‘LA-716’ accession confer good levels of resistance to arthropod pests. The objective of the present study was to select F2 plants from the interspecific cross Solanum pennellii ‘LA-716’ x Solanum lycopersicum ‘Redenção’ to assess resistance to spider .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.8
- DOI:
- 10.4238/2015.January.16.8
Changes in gene expression profiles of bovine embryos produced in vitro, by natural ovulation, or hormonal superstimulation
Authors: T.C.D. Mundim, A.F. Ramos, R. Sartori, M.A.N. Dode, E.O. Melo, L.F.S. Gomes, R. Rumpf and M.M. Franco
Embryos produced by hormonal superstimulation have been used as an in vivo control in most published research on embryo gene expression. However, it is not known if this is the most appropriate control for gene expression profile studies. We compared the expression of GRB-10, IGF-II, IGF-IIR, MnSOD, GPX-4, catalase, BAX, and i.. Read More»
- Genet. Mol. Res. 8(4):
- vol8-4gmr646
- DOI:
- 10.4238/vol8-4gmr646
Molecular conservation of the mammalian leptin protein
Authors: J.E. Gabriel and K.C.F. Lidani
In this study, we comparatively assessed multiple sequences of the leptin protein from different animal species to establish new insights into conservation degree of biological sequences and evolutionary biology among mammals using computational biology tools. First, amino acid sequences of the leptin protein from Homo sapiens (human, P41159), Sus scrofa .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.16.9
- DOI:
- 10.4238/2015.January.16.9
Population structure of rice varieties used in Turkish rice breeding programs determined using simple-sequence repeat and inter-primer binding site-retrotransposon data
Authors: G. C�¶mertpay1, F.S. Baloch2, M. Derya3, E.E. Andeden3, A. Alsaleh3, H. S�¼rek4 and H. ��zkan5
Abstract Effective breeding programs based on genetic diversity are needed to broaden the genetic basis of rice (Oryza sativa L.) in Turkey. In this study, 81 commercial varieties from seven countries were studied in order to estimate the genomic relationships among them using nine inter-primer binding site (iPBS)-retrotransposon and 17 simple-sequence r.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017158
- DOI:
- 10.4238/gmr.15017158