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Cytogenomics   Research Article

Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Authors: Z. Noormohammadi Y. Hasheminejad-Ahangarani Farahani M. Sheidai S. Ghasemzadeh-Baraki O. Alishah

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD.. Read More»

Genet. Mol. Res. 12(1):
2013.January.30.12
DOI:
10.4238/2013.January.30.12
Animal Genetics   Research Article

Internal phloem in an interspecific hybrid of cassava, an indicator of breeding value for drought resistance

Authors: D. Graciano-Ribeiro, D.Y.C. Hashimoto, L.C. Nogueira, D. Teodoro, S.F. Miranda and N.M.A. Nassar

We examined the stem anatomy of Manihot esculenta Crantz and its hybrid with M. oligantha Pax. Cross-sections were stained with safranin-alcian blue. Manihot esculenta and its hybrid were found to have the same vascular structure in bicollateral bundles with internal phloem, which has been associated with drought resistance in.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr629
DOI:
10.4238/vol8-3gmr629
Microbial Genetics   Research Article

Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers

Authors: R.G.F. Morales J.T.V. Resende F.V. Resende C.A. Delatorre A.S.T. Figueiredo P.R. Da-Silva

Outside its centers of origin, garlic propagates only asexually. Since asexual reproduction leads to the absence of meiotic recombination, the main garlic cultivars available for cultivation have arisen from the accumulation of somatic mutations in early cultivars. Thus, it is common for a single clone to have different names in different regions. This st.. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.1
DOI:
10.4238/2013.February.4.1
Animal Genetics   Research Article

Oropharyngeal dysphagia and language delay in partial trisomy 9p: case report

Authors: N.F. Rossi, A.R. Gatto, P.C. Cola, D.H. Souza, D. Moretti-Ferreira and C.M. Giacheti

The phenotype of partial trisomy 9p includes global developmental delay, microcephaly, bulbous nose, downturned oral commissures, malformed ears, hypotonia, and severe cognitive and language disorders. We present a case report and a comparative review of clinical findings on this condition, focusing on speech-language developm.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr621
DOI:
10.4238/vol8-3gmr621
Human Genetics   Research Article

Corrigendum: Association between TAP1 gene polymorphisms and alopecia areata in a Korean population - Genet. Mol. Res. 14 (4): 18820-18827

Authors: H.K. Kim1*, H. Lee1*, B.L. Lew2, W.Y. Sim2, Y.O. Kim3, S.W. Lee3, S. Lee4, I.H. Cho5, J.T. Kwon1 and H.J. Kim1,6

H.K. Kim1*, H. Lee1*, B.L. Lew2, W.Y. Sim2, Y.O. Kim3, S.W. Lee3, S. Lee4, I.H. Cho5, J.T. Kwon1 and H.J. Kim1,6 5Department of Convergence Medical Science, Brain Korea 21 Plus Program, and Institute of Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea H.K. Kim1*, H. Lee1*, B.L. Lew2, W.Y. Sim2, Y.O. Kim3, S.W. .. Read More»

Genet. Mol. Res. 15(1):
gmr.150170751
DOI:
10.4238/gmr.150170751
Microbial Genetics   Research Article

Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

Authors: B. Canto-Canch?© M. Tzec-Sim?¡ J.I. V?¡zquez-Lor?­a H. Espadas-?lvarez B.H. Ch?­-Manzanero R. Rojas-Herrera R. Valdez-Ojeda L. Alzate-Gaviria

Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the mi.. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.2
DOI:
10.4238/2013.February.4.2
Bioinformatics   Research Article

Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba

Authors: F. Xu X.H. Huang L.L. Li G. Deng H. Cheng X.F. Rong J.B. Li S.Y. Cheng

Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The .. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.3
DOI:
10.4238/2013.February.4.3
Human Genetics   Research Article

Lenalidomide affect expression level of cereblon protein in multiple myeloma cell line RPMI8226

Authors: D.Y. Yang, J.H. Ren, X.N. Guo, X.L. Guo, X.Y. Cai, X.F. Guo and J.N. Zhang

We investigated the mechanisms of action of immunomodulatory drug (lenalidomide) on the protein expression of cereblon (CRBN) and their therapeutic targets in the multiple myeloma cell line RPMI8226. The multiple myeloma cell line RPMI8226 was cultured and treated with different concentrations of lenalidomide and bortezomib to determine the proliferation .. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.19
DOI:
10.4238/2015.October.28.19
Human Genetics   Research Article

An alternate method for DNA and RNA extraction from clotted blood

Authors: Z. Zakaria S.H. Umi S.S. Mokhtar U. Mokhtar M.Z. Zaiharina A.T.A. Aziz B.P. Hoh

We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were a.. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.4
DOI:
10.4238/2013.February.4.4
Plant Genetics   Research Article

Antimicrobial potential of actinobacteria isolated from the rhizosphere of the Caatinga biome plant Caesalpinia pyramidalis Tul.

Authors: G.R. Silva-Lacerda, R.C.F. Santana, M.C.V. Vicalvi-Costa, E.G. Solid?´nio, K.X.F.R. Sena, G.M.S. Lima and J.M. Ara?ºjo

Actinobacteria are known to produce various secondary metabolites having antibiotic effects. This study assessed the antimicrobial potential of actinobacteria isolated from the rhizosphere of Caesalpinia pyramidalis Tul. from the Caatinga biome. Sixty-eight actinobacteria isolates were evaluated for antimicrobial activity against different microorganisms .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017488
DOI:
10.4238/gmr.15017488