Leaf carbon assimilation and molecular phylogeny in Cattleya species (Orchidaceae)
Authors: V. Andrade-Souza, A.-A.F. Almeida, R.X. Corr�ªa, M.A. Costa,\M.S. Mielke and F.P. Gomes
We examined leaf CO2 assimilation and how it varied among species within the orchid genus Cattleya. Measurements of CO2 assimilation and maximum quantum yield of PS II (Fv/Fm) were made for mature leaves of nine species using a portable system for photosynthesis measurement and a portable fluorometer. Leaf area was measured with an area meter, and th.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr618
- DOI:
- 10.4238/vol8-3gmr618
SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon
Authors: G. Li Y.-J. Park
The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to de.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.14
- DOI:
- 10.4238/2012.May.18.14
Identification of the e allele at the Extension locus (MC1R) in Brazilian Creole sheep and its role in wool color variation
Authors: D. Hepp G.L. Gon�§alves G.R.P. Moreira T.R.O. Freitas C.T.D.C. Martins T.A. Weimer D.T. Passos
The melanocortin 1 receptor (MC1R) gene has been described as responsible for the black color in some breeds of sheep, but little is known about its function in many colored breeds, particularly those with a wide range of pigmentation phenotypes. The Brazilian Creole is a local breed of sheep from southern Brazil that has a wide variety of wool colors. We.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.5
- DOI:
- 10.4238/2012.May.22.5
In vitro regeneration of cocona (Solanum sessiliflorum, Solanaceae) cultivars for commercial production
Authors: A.R. Schuelter1, A.K. Grunvald1, A.T. Amaral J�ºnior2, C.L. da Luz1,C.L. Luz1, L.M. Gon�§alves1, S. Stefanello1 and C.A. Scapim3
Cocona (Solanum sessiliflorum Dunal) is a solanaceous shrub native to the Amazon region that produces an edible fruit. This species has numerous advantages, particularly a high nutritional value and productivity. However, due to irregular germination and rapid loss of seed viability, there are few plantations for production on a large scale. Development o.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr613
- DOI:
- 10.4238/vol8-3gmr613
Genotyping of urinary samples stored with EDTA for forensic applications
Authors: S.H. Zhang S.M. Zhao Z.M. Zhao C.T. Li
Individual identification of urinary samples is necessary when sample switching or handling are suspected during a judicial process. To improve the rate of successful genotyping of urinary samples, we examined the stability of DNA in urinary samples stored for up to 30 days. Urinary samples from 20 healthy individuals (10 males and 10 females) were stored.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.10.5
- DOI:
- 10.4238/2012.May.10.5
Characterization of novel nitrate reductase-deficient mutants for transgenic Dunaliella salina systems
Authors: L.J. Gao, Y.L. Jia, S.K. Li and L.L. Qiu
The aim of the present study was to isolate and characterize novel nitrate reductase (NR)-deficient mutants, which may be useful for the transgenic manipulation of Dunaliella salina. Three NR-deficient mutants of D. salina, J-1, J-2, and J-3, were successfully isolated by screening for chlorate resistance after chemical mutagenesis with ethylnitrosourea. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.25
- DOI:
- 10.4238/2015.October.26.25
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
Authors: N.B. Stafuzza C.A. Abbey C.A. Gill J.E. Womack M.E.J. Amaral
River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC li.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.6
- DOI:
- 10.4238/2012.May.22.6
Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility
Authors: M.H. Deng J.F. Wen J.L. Huo H.S. Zhu X.Z. Dai Z.Q. Zhang H. Zhou X.X. Zou
NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nuc.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.15
- DOI:
- 10.4238/2012.May.18.15
Genetic diversity of mitochondrial control region (D-Loop) polymorphisms in Coilia ectenes taihuensis inhabiting Taihu Lake, China
Authors: J.J. Zhang, J.R. Duan, Y.F. Zhou, J.Y. Peng, D.A. Fang
Coilia ectenes is a commercially important fishery species in China. C. ectenes taihuensis is an endemic and dominant species found in Taihu Lake of China. When compared with C. ectenes, C. ectenes taihuensis lacks anadromous behavior, and can independently grow and reproduce in Taihu Lake. In this study, the mitochondrial DNA.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019457
- DOI:
- 10.4238/gmr16019457
A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers
Authors: Y.P. Zhang H.H. Tan S.Y. Cao X.C. Wang G. Yang J.G. Fang
DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plan.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.30.1
- DOI:
- 10.4238/2012.May.30.1