Table of Contents | Genet. Mol. Res.2012(3)
Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)
Authors: J.Y. Zheng, H. Wang, X.X. Chen, P. Wang, P. Gao, X.N. Li and G.P. Zhu
Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellit.. Read More»
Genet. Mol. Res. 11(3): 2012.August.6.1
DOI: 10.4238/2012.August.6.1
Detection of bacterial blight resistance genes in basmati rice landraces
Authors: I. Ullah, S. Jamil, M.Z. Iqbal, H.L. Shaheen, S.M. Hasni, S. Jabeen, A. Mehmood and M. Akhter
Aromatic basmati rice is vulnerable to bacterial blight disease. Genes conferring resistance to bacterial blight have been identified in coarse rice; however, their incorporation into basmati varieties compromises the prized basmati aroma. We identified bacterial blight resistance genes Xa4, xa5, Xa7, and xa13 in 52 basmati la.. Read More»
Genet. Mol. Res. 11(3): 2012.July.20.1
DOI: 10.4238/2012.July.20.1
A family-based association study identified CYP17 as a candidate gene for obesity susceptibility in Caucasians
Authors: H. Yan, Y. Guo, T.-L. Yang, L.-J. Zhao and H.-W. Deng
The cytochrome P450c17α gene (CYP17) encodes a key biosynthesis enzyme of estrogen, which is critical in regulating adipogenesis and adipocyte development in humans. We therefore hypothesized that CYP17 is a candidate gene for predicting obesity. In order to test this hypothesis, we performed a family-based association t.. Read More»
Genet. Mol. Res. 11(3): 2012.May.22.1
DOI: 10.4238/2012.May.22.1
An oleosin-fusion protein driven by the CaMV35S promoter is accumulated in Arabidopsis (Brassicaceae) seeds and correctly targeted to oil bodies
Authors: W. Li, L.G. Li, X.F. Sun1and K.X. Tang
Oleosin-fusion technology is used to express desired proteins. It was developed based on the properties of oleosin; the heterologous protein gene is fused to the oleosin gene and the fusion gene is driven by a seed-specific promoter. We replaced the seed specific promoter with the CaMV35S promoter to dive a gfp-oleosin fusion gene in transformed Arabidops.. Read More»
Genet. Mol. Res. 11(3): 2012.August.13.1
DOI: 10.4238/2012.August.13.1
Complete sequence analysis of mitochondrial DNA of aplastic anemia patients
Authors: X. Cui, F. Liu, J.Q. Wang, W.J. Zhang, J.Y. Wang, K. Liu, S.Y. Cui, J. Zhang and R.R. Xu
This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients’ oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by .. Read More»
Genet. Mol. Res. 11(3): 2012.August.6.16
DOI: 10.4238/2012.August.6.16
Gene expression and molecular modeling of the HSP104 chaperone of Trypanosoma cruzi
Authors: R.A. Campos, M.L. da Silva, G.V. da Costa, P.M. Bisch, J.M. Peralta, R. Silva, E. Rondinelli and T.P. ��rm�©nyi
Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a si.. Read More»
Genet. Mol. Res. 11(3): 2012.August.6.15
DOI: 10.4238/2012.August.6.15
Comparison of the prevalence and distribution of human papillomavirus infection and cervical lesions between urban and native habitants of an Amazonian region of Peru
Authors: M. Martorell, J.A. Garcia-Garcia, D. Gomez-Cabrero, A. del Aguila
We made a study of the prevalence of human papillomavirus (HPV) and cervix lesions in an Amazonian Bora native population (Bn) and compared it with the prevalence in an urban population in Iquitos (Iq). We also examined the distribution of HPV types among abnormal cervical smears in the Iq population. Swabs and cytologies were collected from 472 females. .. Read More»
Genet. Mol. Res. 11(3): 2012.August.6.14
DOI: 10.4238/2012.August.6.14
Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa
Authors: W.J. Li, H. Ma, Z.H. Li, Y.M. Wan, X.X. Liu and C.L. Zhou
We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism... Read More»
Genet. Mol. Res. 11(3): 2012.August.6.13
DOI: 10.4238/2012.August.6.13