G.S. Silva
Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding
The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant.. Read More»
Genet. Mol. Res. 16(3): gmr16039582
DOI: 10.4238/gmr16039582
Analysis of transferability of microsatellite primers (SSR) in wild Passiflora species and intraspecific genetic diversity in Passiflora alata
The genus Passiflora L. is the most representative of Passifloraceae, with over 500 known species, among which 150-200 originated from Brazil. In addition to the great commercial importance of this genus for the fruit market, many of the species have exotic flowers with a huge diversity of colors and can thereby be exploited as ornamental plants. This study.. Read More»
Genet. Mol. Res. 13(3): 2014.August.7.6
DOI: 10.4238/2014.August.7.6
Genomic in situ hybridization in plants
Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool h.. Read More»
Genet. Mol. Res. 12(3): 2013.August.12.11
DOI: 10.4238/2013.August.12.11
Lactic acid bacteria dynamics during spontaneous fermentation of cocoa beans verified by culture-independent denaturing gradient gel electrophoresis
Cocoa is naturally fermented in the field before the cocoa seeds are removed for processing. We assessed the dynamics of lactic acid bacteria during cocoa fermentation in Bahia, Brazil. During five days of fermentation, temperature and pH were measured and beans were collected for genomic DNA extraction every 12 h. The DNA was used as a template for amplification wi.. Read More»
Genet. Mol. Res. 10(4): 2011.November.4.3
DOI: 10.4238/2011.November.4.3
Effect of organic matter enrichment on the fungal community in limestone cave sediments
Caves are considered major touristic attractions. The management plans of many such caves include limiting the number of visitors; however, the human impact on microbial communities within caves is rarely considered. Therefore, the aim of this study was to evaluate the impact of human-transferred organic matter on the fungal microcosms growing on cave sediments. Sam.. Read More»
Genet. Mol. Res. 15(3): gmr.15038611
DOI: 10.4238/gmr.15038611
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prepared with the DNA from the paternal geni.. Read More»
Genet. Mol. Res. 14(1): 2015.March.27.4
DOI: 10.4238/2015.March.27.4
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