Plant Genetics
Expression analysis of dihydroflavonol 4-reductase genes in Petunia hybrida
Authors: Y.X. Chu, H.R. Chen, A.Z. Wu, R. Cai and J.S. Pan
Dihydroflavonol 4-reductase (DFR) genes from Rosa chinensis (Asn type) and Calibrachoa hybrida (Asp type), driven by a CaMV 35S promoter, were integrated into the petunia (Petunia hybrida) cultivar 9702. Exogenous DFR gene expression characteristics were similar to flower-color changes, and effects on anthocyanin concentration were observed in both types .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.12.4
- DOI:
- 10.4238/2015.May.12.4
The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
Authors: A.C.G. Moura, F.F. Perazzo and E.L. Maistro
We examined the genotoxic and mutagenic effects of a crude extract of Clusia alata (a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was in.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr523
- DOI:
- 10.4238/vol7-4gmr523
Assessment of genetic diversity and variation of Robinia pseudoacacia seeds induced by short-term spaceflight based on two molecular marker systems and morphological traits
Authors: C.Q. Yuan Y.F. Li P. Sun Y.H. Sun G.J. Zhang M.S. Yang Y.Y. Zhang Y. Li L. Wang
The black locust (Robinia pseudoacacia) is a forest legume that is highly valued as a honey plant and for its wood. We explored the effect of short-term spaceflight on development of R. pseudoacacia seedlings derived from seeds that endured a 15-day flight; the genetic diversity and variation of plants sampled from space-mutagenized seeds were compared to pl.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.December.17.2
- DOI:
- 10.4238/2012.December.17.2
Flow cytometry for diepoxybutane test analysis
Authors: C.F.A. Moreira, L.C. Brito J�ºnior and J.A.R. Lemos
The main feature of Fanconi anemia (FA) is the high sensitivity of the cells to the clastogenic agent, diepoxybutane (DEB). Thus, differential diagnosis of this syndrome can be made by cytogenetic analysis; adding DEB to lymphocytes in culture (DEB test) increases the number of chromosome breaks. Fanconi anemia cells have an a.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr510
- DOI:
- 10.4238/vol7-4gmr510
Genetic diversity of Phytophthora capsici (Pythiaceae) isolates in Anhui Province of China based on ISSR-PCR markers
Authors: P. Li S. Cao Y.L. Dai X.L. Li D.F. Xu M. Guo Y.M. Pan Z.M. Gao
Phytophthora capsici is a plant pathogenic oomycete that damages numerous crops worldwide. Consequently, interest in research on the genetic structure of this species has grown in recent decades. However, there is little information about P. capsici in eastern China. We investigated the genetic diversity of P. capsici isolates from three large regions of .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.December.17.4
- DOI:
- 10.4238/2012.December.17.4
Isolation and characterization of novelEST-SSRs in the showy dendrobium, Dendrobium nobile (Orchidaceae)
Authors: J.J. Lu, J.Y. Kang, S.R. Ye and H.Z. Wang
Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers could enrich the current resource of molecular markers. In this study, microsatellite markers were developed for Dendrobium nobile Lindl. by mining the ESTs. Twenty-eight EST-SSRs amplified 2 to 6 nucleotide repeats with a mean number of 2.82 alleles per.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.10
- DOI:
- 10.4238/2014.February.19.10
Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds
Authors: H.O. Santos, E.V.R. Von Pinho, I.V. Von Pinho, S.M.F. Dutra,T. Andrade and R.M. Guimar�£es
Phytohormones have different characteristics and functions, and they may be subject to changes in their gene expression and synthesis during seed development. In this study, we evaluated the physiological qualities of habanero peppers (Capsicum chinense Jacquin) during seed development and the expression of genes involved in germination. Seeds were obtain.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.12.11
- DOI:
- 10.4238/2015.May.12.11
Analysis of persistent changes to �³-aminobutyric acid receptor gene expression in Plutella xylostella subjected to sublethal amounts of spinosad
Authors: X.-H. Yin, Q.-J. Wu, Y.-J. Zhang, Y.-H. Long, X.-M. Wu, R.-Y. Li, M. Wang, X.-L. Tian and X.-G. Jiao
A multi-generational approach was used to investigate the persistent effects of a sub-lethal dose of spinosad in Plutella xylostella. The susceptibility of various sub-populations of P. xylostella to spinosad and the effects of the insecticide on the gene expression of γ-aminobutyric acid receptor (GABAR) were determined. The results of a leaf dip b.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038782
- DOI:
- 10.4238/gmr.15038782
Genetic divergence of common bean cultivars
Authors: J.S. Veloso, W. Silva, L.R. Pinheiro, J.B. dos Santos, N.S. Fonseca Jr. and M.P. Euzebio
The aim of this study was to evaluate genetic divergence in the ‘Carioca’ (beige with brown stripes) common bean cultivar used by different institutions and in 16 other common bean cultivars used in the Rede Cooperativa de Pesquisa de Feijão (Cooperative Network of Common Bean Research), by using simple sequence repeats associated with .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.22
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.22
Mechanism of juglone-induced apoptosis of MCF-7 cells by the mitochondrial pathway
Authors: Y.B. Ji, G.S. Xin, Z.Y. Qu, X. Zou and M. Yu
This study investigated the nature and mechanism of juglone-induced apoptosis in the human breast cancer cell line MCF-7. The inhibitory effect of juglone on MCF-7 cell growth was evaluated by the dimethylthiazol tetrazolium assay. Morphological apoptotic changes were characterized using an inverted microscope, Hoechst 33258 fluorescence staining, and Gie.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038785
- DOI:
- 10.4238/gmr.15038785