Plant Genetics
Transgenic rice expressing a cassava (Manihot esculenta Crantz) plasma membrane gene MePMP3-2 exhibits enhanced tolerance to salt and drought stresses
Authors: Y. Yu1,2*, Y.C. Cui1*, C. Ren1,2, P.S.C.F. Rocha1, M. Peng3, G.Y. Xu4, M.L. Wang1 and X.J. Xia1
Plasma membrane proteolipid 3 (PMP3) is a class of small hydrophobic proteins found in many organisms including higher plants. Some plant PMP3 genes have been shown to respond to abiotic stresses and to participate in the processes of plant stress tolerance. In this study, we isolated the cassava (Manihot esculenta Crantz) MePMP3-2 gene and functionally c.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017336
- DOI:
- 10.4238/gmr.15017336
Assessing molecular and morpho-agronomical diversity and identification of ISSR markers associated with fruit traits in quince (Cydonia oblonga)
Authors: I. Ganopoulos, G. Merkouropoulos, S. Pantazis, C. Tsipouridis and A. Tsaftaris
Quince is a deciduous tree known to the countries around the Mediterranean since antiquity. Nowadays, quince is used as an ornamental plant, and as a rootstock for pear trees, with its fruit being appreciated mainly for production of jam and sweets rather than for raw consumption. Quince leaves contain compounds with antioxida.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.4.7
- DOI:
- 10.4238/2011.November.4.7
Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri
Authors: A. Soares-Costa R.S. Silveira M.T.M. Novo M.F.M. Alves A.K. Carmona J. Belasque Jr. F. Henrique-Silva
Xanthomonas citri subsp citri (Xac) is the bacterium responsible for citrus canker disease in citrus plants. The aim of this study was to describe the recombinant expression, purification, and characterization of a cysteine peptidase from Xac strain 306, which is a candidate for involvement in the pathogenicity of this bacterium. The gene was cloned and expr.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.3
- DOI:
- 10.4238/2012.November.28.3
Variability and inbreeding in semiexotic maize populations
Authors: A.S. Oliveira, J.B. Miranda Filho and E.F. Reis
Three semiexotic populations (CRE-01, CRE-02, CRE-03) obtained by incorporation of exotic germplasm (lines from CIMMYT, Colombia; selected for resistance to corn stunt complex) were evaluated in two cycles of recurrent selection with half-sib families. In cycle-I, samples comprising 50, 70, and 50 half-sib families were evaluated at Jataí (GO) and .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.21
- DOI:
- 10.4238/2015.February.6.21
Genomic DNA extraction from medicinal plants available in Malaysia using a TriOmicTM improved extraction kit
Authors: A.R. Mohd-Hairul, A.B. Sade, B.C. Yiap and A.R. Raha
DNA extraction was carried out on 32 medicinal plant samples available in Malaysia using the TriOmicTM extraction kit. Amounts of 0.1 g flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1plus buffer and followed by RNAse treatment. Then, RY2 buffer was added to the samples and mixed completely by.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.8.1
- DOI:
- 10.4238/2011.November.8.1
Transcriptome analysis of Arabidopsis seedlings responses to high concentrations of glucose
Authors: L. Han, J.L. Li, M. Jin and Y.H. Su
Sugars acting as fuel energy or as signaling molecules play important roles in plant growth and development. Although sugars associated with early seedling development have been analyzed in detail, few studies have examined the effect of sugar on genome-wide gene transcription. To analyze the role of glucose on the genomic level, we examined the response .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.11
- DOI:
- 10.4238/2015.May.11.11
Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA
Authors: Iqbal A, Khan AS, Khan IA, Awan FS, Ahmad A and Khan AA
The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polym.. Read More»
- Genet. Mol. Res. 6(3):
Evaluation of genetic diversity in a natural rosewood population (Dalbergia nigra Vell. Allemao ex Benth.) using RAPD markers
Authors: Juchum FS, Leal JB, Santos LM, Almeida MP, Ahnert D and Correa RX
Dalbergia nigra (rosewood) is a long-lived leguminous species, which is endemic to the Brazilian Atlantic forest. Because of the high economic value of its wood, this species has been over-explored in recent years. Currently, rosewood is included in the IUCN Red List as vulnerable. We examined the genetic diversity of 87 speci.. Read More»
- Genet. Mol. Res. 6(3):
Next-generation sequencing identification and characterization of microsatellite markers in Aconitum austrokoreense Koidz., an endemic and endangered medicinal plant of Korea
Authors: Y.-E. Yun, J.-N. Yu, G.H. Nam, S.-A. Ryu, S. Kim, K. Oh and C.E. Lim
We used next-generation sequencing to develop 9 novel microsatellite markers in Aconitum austrokoreense, an endemic and endangered medicinal plant in Korea. Owing to its very limited distribution, over-harvesting for traditional medicinal purposes, and habitat loss, the natural populations are dramatically declining in Korea. All novel microsatellite mark.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.13
- DOI:
- 10.4238/2015.May.11.13
Identification of highly expressed host microRNAs that respond to white spot syndrome virus infection in the Pacific white shrimp Litopenaeus vannamei (Penaeidae)
Authors: D.G. Zeng, X.L. Chen, D.X. Xie, Y.Z. Zhao, Q. Yang, H. Wang, Y.M. Li and X.H. Chen
MicroRNAs (miRNAs) are known to play an important role in regulating both adaptive and innate immunity. Pacific white shrimp (Litopenaeus vannamei) is the most widely farmed crustacean species in the world. However, little is known about the role miRNAs play in shrimp immunity. To understand the impact of viral infection on miRNA expression in shrimp, we .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.14
- DOI:
- 10.4238/2015.May.11.14