Plant Genetics
Isolation and characterization of microsatellite markers in Atrina vexillum Born
Authors: Y.F. Ning, Z.B. Li, Y.S. Huang, X.Q. Mao, B.B. Li, Y. Yuan and J.B. Shangguan
Atrina vexillum Born is an economically valuable species, widely distributed in the coastal waters of temperate and tropical areas of the Asia Pacific region. Twenty one novel microsatellite loci were identified in the genome of A. vexillum Born using the protocol for fast isolation by amplified fragment length polymorphism of.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027204
- DOI:
- 10.4238/gmr.15027204
Assessment of genetic relationship in Persea spp by traditional molecular markers
Authors: J.C. Reyes-Alem�¡n, E. Valadez-Moctezuma and A.F. Barrientos-Priego
Currently, the reclassification of the genus Persea is under discussion with molecular techniques for DNA analysis representing an alternative for inter- and intra-specific differentiation. In the present study, the traditional random-amplified polymorphic DNA (RAPD) and the inter simple sequence repeat (ISSR) markers were use.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027359
- DOI:
- 10.4238/gmr.15027359
Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing
Authors: H. Liu, Z.C. Long, L.N. Li, Q.F. Wang, J.M. Chen and K. Liao
To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The numbe.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027886
- DOI:
- 10.4238/gmr.15027886
Cross-species amplification and polymorphism of microsatellite loci in Helicoverpa armigera and Helicoverpa zea (Lepidoptera: Noctuidae) in Brazilian cropping systems
Authors: N.A. Leite, A.S. Corr�ªa, A. Alves-Pereira, J.B. Campos, M.I. Zucchi and C. Omoto
The Old World bollworm Helicoverpa armigera (Hübner) was recently discovered in Brazil. This species is closely related to the New World bollworm H. zea (Boddie), and mating between these species has already been reported under laboratory conditions. Here, we tested the cross-species amplification of 20 microsatellite (SS.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027890
- DOI:
- 10.4238/gmr.15027890
Genetic variability and diversity of the main resources of lily assessed via phenotypic characters, pollen morphology, and ISSR markers
Authors: J.M. Wang, S.L.Y. Ma, W.Q. Li, Q. Wang, H.Y. Cao, J.H. Gu and Y.M. Lu
Lily (Lilium spp), which belongs to Lilium, is one kind of monocotyledon. As a perennial ornamental plant with extremely high esthetic, edible, and medicinal value, lily has gained much favor due to its mostly showy flowers of various colors and elegant shape. In this research, we studied experimental materials in a sample of .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027638
- DOI:
- 10.4238/gmr.15027638
Acyl sugars and whitefly (Bemisia tabaci) resistance in segregating populations of tomato genotypes
Authors: D.M. Dias, J.T.V. Resende, J.C. Marodin, R. Matos, I.F. Lustosa and N.C.V. Resende
The wild tomato, Solanum pennellii, is an important source of resistance genes against tomato pests. This resistance is due to the presence of acyl sugars (AS), which are allelochemicals that have negative effects on arthropod pests. There are no commercially available tomato cultivars that exhibit significant levels of resist.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027788
- DOI:
- 10.4238/gmr.15027788
Development of retrotransposon-based markers IRAP and REMAP for cassava (Manihot esculenta)
Authors: B.C. Kuhn, C.A. Mangolin, E.R. Souto, C.M. Vicient and M.F.P.S. Machado
Retrotransposons are abundant in the genomes of plants. In the present study, inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers were developed for the cassava genome (Manihot esculenta Crantz). Four cassava cultivars (Fécula Branca, IPR-Uni&atil.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027149
- DOI:
- 10.4238/gmr.15027149
Variability of Colletotrichum spp in common bean
Authors: S.F. Mota, Q.L. Barcelos, M.A. Dias and E.A. Souza
The Colletotrichum genus presents large genetic variability, as demonstrated by the occurrence of several pathogenic races and phenotypic traits. The objective of this study was to characterize 22 strains of C. lindemuthianum and Colletotrichum spp recovered from anthracnose lesions and bean scab, and to verify the relationshi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027176
- DOI:
- 10.4238/gmr.15027176
Genetic analysis of floral organ size in broccoli Ã?Â? cabbage via a mixed inheritance model of a major gene plus polygene
Authors: J.S. Shu, Y.M. Liu, Z.S. Li, L.L. Zhang, Z.Y. Fang, L.M. Yang, M. Zhuang,Y.Y. Zhang and H.H. Lv
Broccoli and cabbage are important vegetable crops that produce hybrid seeds after insect pollination; the size of floral organs is crucial for this process. To investigate the genetic characteristics of floral organ sizes (corolla width, petal length and width, and lengths of stamen, anther, style, and stigma) and to improve .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027554
- DOI:
- 10.4238/gmr.15027554
Comparative analysis of protocols for DNA extraction from soybean caterpillars
Authors: J. Palma, I. Valmorbida, I.F.D. da Costa and J.V.C. Guedes
Genomic DNA extraction is crucial for molecular research, including diagnostic and genome characterization of different organisms. The aim of this study was to comparatively analyze protocols of DNA extraction based on cell lysis by sarcosyl, cetyltrimethylammonium bromide, and sodium dodecyl sulfate, and to determine the most.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027904
- DOI:
- 10.4238/gmr.15027904