Plant Genetics
Improved production of transgenic Dioscorea zingiberensis (Dioscoreaceae) by Agrobacterium tumefaciens-mediated transformation
Authors: L. Shi, J.Q. Fan, C.G. Hu, J. Luo and J.L. Yao
The establishment of high-efficiency Agrobacterium-mediated transformation techniques could improve the production of Dioscorea zingiberensis, a medicinal species with a high diosgenin content. We co-cultivated embryogenic calli induced from mature seeds with A. tumefaciens strain EHA105. A binary vector, pCAMBIA1381, which co.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.3.4
- DOI:
- 10.4238/2012.February.3.4
Allele frequencies for 12 autosomal short tandem repeat loci in two bolivian populations
Authors: L. Cifuentes, H. Jorquera, M. Acu�±a, J. Ord�³�±ez and A.L. Sierra
Two hundred and sixty unrelated subjects who asked for paternity testing at two Bolivian Laboratories in La Paz and Santa Cruz were studied. The loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, and CSF1PO were typed from blood samples, amplifying DNA by polymerase chain reactions and electr.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr368
- DOI:
- 10.4238/vol7-1gmr368
Assessment of genetic diversity in lentils (Lens culinaris Medik.) based on SNPs
Authors: R. Basheer-Salimia, B. Camilli, S. Scacchi, E. Noli andM. Awad
This study is the first attempt to establish an SNP database for the purpose of estimating the genetic diversity and relatedness of Palestinian lentil genotypes. A total of 14 lentil accessions (11 local, two supplied by ICARDA, and one introduced from Italy) were investigated. By sequencing two genes, lectin and lipid transfer protein 5 (LTP5), four SNPs.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.4
- DOI:
- 10.4238/2015.June.1.4
Leaf morphological and genetic divergence in populations of Drimys (Winteraceae) in Chile
Authors: P. Jara-Arancio, M.R. Carmona, C. Correa, F.A. Squeo and G. Arancio
The genus Drimys is distributed in Chile from semi-arid zones to sub-Antarctic forests; there are three species of this tree, D. andina, D. confertifolia and D. winteri, the latter with varieties chilensis and winteri. Northern populations are found in small disjunct natural refuges, specifically mountain cloud forests and the.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.3.3
- DOI:
- 10.4238/2012.February.3.3
A description of genes of Corynebacterium pseudotuberculosis useful in diagnostics and vaccine applications
Authors: V. D�Afonseca, P.M. Moraes, F.A. Dorella, L.G.C. Pacheco,R. Meyer, R.W. Portela, A. Miyoshi* and V. Azevedo*
Corynebacterium pseudotuberculosis, a Gram-positive intracellular pathogen, is the etiological agent of caseous lymphadenitis or CLA. This bacterium infects goats and sheep and causes great economic losses worldwide annually, mainly for goat producers. Despite its importance, CLA is still poorly characterized. However, with ad.. Read More»
- Genet. Mol. Res. 7(1):
Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bos taurus x Bos indicus beef composite population
Authors: E.P. Marson, J.B.S. Ferraz, F.V. Meirelles, J.C.C. Balieiroand J.P. Eler
The purpose of the present research was to investigate the effects of polymorphisms of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes, evaluated by polymerase chain reaction-restriction fragment length polymorphism in European-Zebu composite beef heifers from six different breed compo.. Read More»
- Genet. Mol. Res. 7(1):
Genetic diversity of root anatomy in wild and cultivated Manihot species
Authors: N.N. Bomfim, D. Graciano-Ribeiro and N.M.A. Nassar
An anatomical study of roots was conducted on two wild Manihot species, namely M. glaziovii and M. fortalezensis, and two cassava varieties, M. esculenta Crantz variety UnB 201 and M. esculenta variety UnB 122, to identify taxonomic differences in primary growth. Anatomical characters of cassava roots have been rarely investig.. Read More»
- Genet. Mol. Res. 10(2):
Prognostic value of TP53 Pro47Ser and Arg72Pro single nucleotide polymorphisms and the susceptibility to gliomas in individuals from Southeast Brazil
Authors: G.R. Pinto, F.K.N. Yoshioka, R.L.L. Silva, C.A. Clara, M.J. Santos, J.R.W. Almeida, R.R. Burbano, J.A. Rey and C. Casartelli
The TP53 tumor suppressor gene codifies a protein responsible for preventing cells with genetic damage from growing and dividing by blocking cell growth or apoptosis pathways. A common single nucleotide polymorphism (SNP) in TP53 codon 72 (Arg72Pro) induces a 15-fold decrease of apoptosis-inducing ability and has been associat.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr415
- DOI:
- 10.4238/vol7-1gmr415
Isolation and characterization of the organ-specific and light-inducible promoter of the gene encoding rubisco activase in potato (Solanum tuberosum)
Authors: D. Qu, Y. Song, W.M. Li, X.W. Pei, Z.X. Wang, S.R. Jia and Y.Q. Zhang
Constitutive promoters have been widely used in crop biotechnology applications. Tissue-specific or inducible promoters, however, have advantages in some cases. We isolated the 731-bp 5' flanking sequence of a potato (Solanum tuberosum) gene, encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase (RCA), wh.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1088
- DOI:
- 10.4238/vol10-2gmr1088
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants
Authors: A.A. Alatar, M.A. Mahmoud, S.A. Al-Sohaibani and K.A. Abd-Elsalam
Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was u.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.13.1
- DOI:
- 10.4238/2012.February.13.1