Plant Genetics
Genetic potential of black bean genotypes with predictable behaviors in multienvironment trials
Authors: P.P. Torga, P.G.S. Melo, H.S. Pereira, L.C. Faria and L.C. Melo
The aim of this study was to evaluate the phenotypic stability and specific and broad adaptability of common black bean genotypes for the Central and Center-South regions of Brazil by using the Annicchiarico and AMMI (weighted average of absolute scores: WAAS, and weighted average of absolute scores and productivity: WAASP) me.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049029
- DOI:
- 10.4238/gmr15049029
Evaluation of reference genes for RT-qPCR studies in the leaves of rice seedlings under salt stress
Authors: G.P. Moraes, L.C. Benitez, M.N. do Amaral, I.L. Vighi, P.A. Auler, L.C. da Maia, V.J. Bianchi and E.J.B. Braga
To obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (RT-qPCR) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions. In this study, the stability of expression was evalu.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.24
- DOI:
- 10.4238/2015.March.27.24
Identification of the transcriptionally active cytochrome P450 repertoire in Coffea arabica
Authors: S.T. Ivamoto, D.S. Domingues, L.G.E. Vieira and L.F.P. Pereira
Cytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many s.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.27.25
- DOI:
- http://dx.doi.org/10.4238/2015.March.27.25
Purification, biochemical characterization, and antimicrobial activity of a new lipid transfer protein from Coffea canephora seeds
Authors: G.C.V. Bard, U. Zottich, T.A.M. Souza, S.F.F. Ribeiro, G.B. Dias, S. Pireda, M. Da Cunha, R. Rodrigues, L.S. Pereira, O.L.T. Machado, A.O. Carvalho and V.M. Gomes
Coffee, an agronomical crop of great economic importance, is also among the most commonly traded commodities in worldwide markets. Antimicrobial peptides, which play a role in plant defense, have been identified and isolated particularly from seeds. We isolated and immunolocalized Cc-LTP2, a new lipid transfer protein (LTP) fr.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048859
- DOI:
- 10.4238/gmr15048859
Plant microsatellite genotyping with 4-color fluorescent detection using multiple-tailed primers
Authors: Alexandre Missiaggia and Dario Grattapaglia
We extended the concept of fluorescent microsatellite genotyping with a single-universal tailed primer to the simultaneous use of three different tailed primers to allow multiplexed 4-color detection for medium throughput genotyping of plant species. The method was tested on Eucalyptus DNA samples using three forward primer se.. Read More»
- Genet. Mol. Res. 5(1):
Using the attract method to identify core pathways in juvenile idiopathic arthritis
Authors: J.S. Li, X.L. Gao, Y.R. Liu and Y. Dong
The aim of this study was to identify core pathways associated with juvenile idiopathic arthritis (JIA) using the attract method. Kyoto Encyclopedia of Genes and Genomes pathways were determined using the GSEA-ANOVA method, based on the gene expression data of JIA. Syn-expression groups within core attractor pathways were identified by hierarchical cluste.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038331
- DOI:
- 10.4238/gmr.15038331
Development and characterization of polymorphic microsatellite markers for Castanopsis hystrix (Fagaceae)
Authors: Y. Jiang, Z.H. Li, J.Y. Zhu and H.L. Liu
Castanopsis hystrix is one of the most important and dominant species in evergreen broad-leaved forests in subtropical China. However, the population of this species undergone severe decline because of deforestation over the past 2 decades. For both conservation and forestry management, it is essential to develop molecular markers for C. hystrix. We ident.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.1
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.1
Effect of ethyl methyl sulfonate concentration and different treatment conditions on germination and seedling growth of the cucumber cultivar Chinese long (9930)
Authors: S.N.M. Shah, Z.-H. Gong, M.H. Arisha, A. Khan and S.-L. Tian
We attempted to create a new germplasm of cucumber cultivar Chinese long (9930) using different doses of ethyl methyl sulfonate (EMS) to induce variability. We tested EMS concentration (0, 0.5, 1.0, 1.5, 2, 3% v/v) with post-treatment (0.1 M Na2S2O3 and water), EMS concentration (0, 0.5, 1.0, 1.5% v/v) over different treatment times (8, 16, 24 h), and EMS.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.2
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.2
Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China
Authors: J. Yu, Y. Wang, L. Peng, M. Ru and Z.S. Liang
Eucommia ulmoides Oliver, one of the tertiary relict species found only in China, is the only extant species of Eucommiaceae. Using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers, we studied the genetic diversity and population genetic structure of 187 accessions from 17 E. ulmoides populations throughout it.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.5
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.5
Conservation implications of the genetic diversity of Gymnospermium microrrhynchum in Korea
Authors: S.H. Lee, M.H. Yeon and J.K. Shim
Gymnospermium microrrhynchum (Berberidaceae) is an ephemeral perennial herb with a limited distributional range in the Baekdudaegan mountain areas of the Korean Peninsula, and is designated a rare plant by the Korean Forest Service. Information about its genetic variation and structure is important for developing successful co.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048843
- DOI:
- 10.4238/gmr15048843