Plant Genetics

DNA barcoding for efficient identification of Ixiolirion species (Ixioliriaceae)

Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang

Ixiolirion is a genus of unresolved taxonomy. DNA barcoding is a technique that allows species identification using standardized DNA sequences. In this study, a total of 23 individuals, representing 2 Chinese Ixiolirion species, were sampled to test the effectiveness of 3 DNA barcodes [internal transcribed spacer (ITS), chlorop.. Read More»

Genet. Mol. Res. 14(1):

2015.March.13.19

DOI:

10.4238/2015.March.13.19

A non-destructive genotyping system from a single seed for marker-assisted selection in watermelon

Authors: G. Meru, D. McDowell, V. Waters, A. Seibel, J. Davis and C. McGregor

Genet. Mol. Res. 12(1):

Selectional versus mutational mechanism underlying genomic features of bacterial strand asymmetry: a case study in Clostridium acetobutylicum

Authors: H.-L. Zhao, Z.-K. Xia, Z.-G. Hua and W. Wei

Strand biases are widespread in bacterial genomes. In this review, we discuss 5 types of bias, including gene orientation, the number of open reading frames, nucleotide composition, substitution rate, and gene length, between leading and lagging strands during replication. For each type of strand bias, related studies were summarized and Clostridium aceto.. Read More»

Genet. Mol. Res. 14(1):

2015.March.20.1

DOI:

10.4238/2015.March.20.1

Complete mitochondrial genome of a natural triploid crucian carp mutant, Carassius auratus var. pingxiangnensis, and phylogenetic analysis of different ploidies in crucian carp

Authors: J.Q. Sheng, J.H. Wang, S.H. He, L.G. Zeng, K. Peng and Y.J. Hong

Carassius auratus var. pingxiangnensis is a natural triploid crucian carp mutant. In order to understand its placement and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The results showed that the mitochondrial DNA is a ci.. Read More»

Genet. Mol. Res. 13(3):

2014.August.1.3

DOI:

10.4238/2014.August.1.3

Purification of the insecticidal Cry2Ad protein from a Bt-isolated BRC-HZP10 strain and its toxin assay to the diamondback moth, Plutella xylostella (L.)

Authors: J.Y. Liao, Y.Q. Gao, Q.Y. Wu, Y.C. Zhu and M.S. You

The present study aims to characterize the Cry2Ad toxin protein isolated from a Bacillus thuringiensis strain, BRC-HZP10, which have a potential insecticidal activity against larvae of the diamondback moth, Plutella xylostella (L.). The crude Bt toxin proteins were isolated and purified by cation exchange chromatography, then equilibrated with 0.2 M NaOH .. Read More»

Genet. Mol. Res. 14(3):

2015.July.13.11

DOI:

10.4238/2015.July.13.11

Minimization of transcriptional temporal noise and scale invariance in the yeast genome

Authors: Ferreira RC, Bosco F, Paiva PB and Briones MRS

The analysis of transcriptional temporal noise could be an interesting means to study gene expression dynamics and stochasticity in eukaryotes. To study the statistical distributions of temporal noise in the eukaryotic model system Saccharomyces cerevisiae, we analyzed microarray data corresponding to one cell cycle for 6200 g.. Read More»

Genet. Mol. Res. 6(2):

Accumulation of flavanols and expression of leucoanthocyanidin reductase induced by postharvest UV-C irradiation in grape berry

Authors: P.F. Wen, W. Ji, M.Y. Gao, T.Q. Niu, Y.F. Xing and X.Y. Niu

To examine the effect of postharvest ultraviolet C (UV-C) irradiation on flavanol polyphenol accumulation in the grape berry, we investigated total flavanol polyphenol content, the enzyme activity of leucoanthocyanidin reductase (LAR), and transcription of Vv lar1 and Vv lar2 using spectrophotometry, real-time polymerase chain reaction, and western blot a.. Read More»

Genet. Mol. Res. 14(3):

2015.July.13.14

DOI:

10.4238/2015.July.13.14

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

Authors: G.Q. Wen, J. Li, X.H. Liu, Y.S. Zhang and S.S. Wen

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (R.. Read More»

Genet. Mol. Res. 13(1):

http://dx.doi.org/2014.February.28.6

DOI:

http://dx.doi.org/10.4238/2014.February.28.6

Variation characteristics of the nitrate reductase gene of key inbred maize lines and derived lines in China

Authors: J.J. Zhang, X.Q. Zhang, Y.H. Liu, H.M. Liu, Y.B. Wang, M.L. Tian and Y.B. Huang

Key inbred lines have played a fundamental role in maize genetics and breeding. Research on variation characteristics of key genes from key inbred lines and from derived lines is important for early identification and evaluation of inbred maize lines. The nitrate reductase (NR) gene, which plays a central role in nitrate acqui.. Read More»

Genet. Mol. Res. 9(3):

vol9-3gmr931

DOI:

10.4238/vol9-3gmr931

Development of novel chloroplast microsatellite markers for Ginkgo biloba

Authors: M. Xu, L.A. Xu, F.L. Cao, H.J. Zhang and F.X. Yu

Ginkgo biloba is considered to be a living fossil that can be used to understand the ancient evolutionary history of gymnosperms, but little attention has been given to the study of its population genetics, molecular phylogeography, and genetic resources assessment. Chloroplast simple sequence repeat (cpSSR) markers are powerful tools for genetic studies .. Read More»

Genet. Mol. Res. 14(3):

2015.July.13.17

DOI:

10.4238/2015.July.13.17