Plant Genetics
Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses (Poaceae)
Authors: L. Yu, X. Wu, X. Tang and B. Yan
cDNA fragments of lea3 genes with a high GC content (from 68 to 77%) were found in several Poaceae, including Sorghum vulgare, Saccharum officinarum, Oryza officinalis, Oryza meyeriana, Ampelocalamus calcareus, Cynodon dactylon, and Zizania latifoli. They were successfully isolated by means of optimal experimental parameters, .. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr852
- DOI:
- 10.4238/vol9-3gmr852
Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae)
Authors: M.A. Lopes, B.T. Hora Junior, C.V. Dias, G.C. Santos, K.P. Gramacho, J.C.M. Cascardo, A.S. Gesteira and F. Micheli
Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches’ broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused o.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr825
- DOI:
- 10.4238/vol9-3gmr825
Genome-wide analysis of cyclins in maize (Zea mays)
Authors: X. Hu, X. Cheng, H. Jiang, S. Zhu, B. Cheng and Y. Xiang
Cyclins are primary regulators of the activity of cyclin-dependent kinases and play crucial roles in cell cycle progression in eukaryotes. Although extensive studies have revealed the roles of some cyclins and underlying mechanisms in plants, relatively few cyclins have been functionally analyzed in maize. We identified 59 cyc.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr861
- DOI:
- 10.4238/vol9-3gmr861
Isolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves
Authors: C.M. RodrÃ?Âguez-GarcÃ?Âa, L. Peraza-EcheverrÃ?Âa, I.R. Islas-Flores, B.B. Canto-CanchÃ?© and R. Grijalva-Arango
High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis. To determine their comparative effectiveness at eliminating polyphenols, polysacch.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr865
- DOI:
- 10.4238/vol9-3gmr865
Resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism
Authors: G.M. Geng, C.C. Zhu and J.Y. Zhou
The resistance of Castanea mollissima Shuhe-WYL strain to Dryocosmus kuriphilus and its molecular mechanism were examined. The larvae of D. kuriphilus were inoculated on the Shuhe-WYL and Qingzha strains, and mortality was observed and compared; the relative mRNA content of the OsCDPK2, receptor-like protein, OsNAC6 protein, KH domain protein, RNA-binding.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.12
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.12
Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis
Authors: F. Ahmad, A.I. Khan, F.S. Awan, B. Sadia, H.A. Sadaqat and S. Bahadur
Genetic diversity analysis of chickpea germplasm can provide practical information for the selection of parental material and thus assist in planning breeding strategies. Chickpea seed is a good source of carbohydrates and proteins, constituting 80% of the total dry seed weight. Released cultivars and advanced lines of 30 chic.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr862
- DOI:
- 10.4238/vol9-3gmr862
Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance
Authors: A.M. Polizel, M.E. Medri, K. Nakashima, N. Yamanaka, J.R.B. Farias, M.C.N. de Oliveira, S.R.R. Marin, R.V. Abdelnoor, F.C. Marcelino-Guimar�£es, R. Fuganti, F.A. Rodrigues, R. Stolf-Moreira, M.A. Beneventi, A.A.P. Rolla, N. Neumaier, K. Yamaguchi-Shinozaki, J.F.C. Carvalho and A.L. Nepomuceno
We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four rand.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.4
- DOI:
- 10.4238/2011.October.21.4
Transcription factors expressed in soybean roots under drought stress
Authors: S.S. Pereira, F.C.M. Guimar�£es, J.F.C. Carvalho, R. Stolf-Moreira, M.C.N. Oliveira, A.A.P. Rolla, J.R.B. Farias, N. Neumaier and A.L. Nepomuceno
To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcript.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.5
- DOI:
- 10.4238/2011.October.21.5
Molecular evolution of two consecutive carotenoid cleavage dioxygenase genes in strigolactone biosynthesis in plants
Authors: R.K. Wang, J.J. Lu, G.N. Xing, J.Y. Gai and T.J. Zhao
Strigolactones are newly discovered plant hormones that perform various functions, from signaling in symbiotic interactions with arbuscular mycorrhizal fungi to controlling outgrowth of axillary buds. We examined the phylogenetic relationships of two carotenoid cleavage dioxygenase genes (CCD7 and CCD8) that are involved in co.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.2.2
- DOI:
- 10.4238/2011.December.2.2
Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)
Authors: J.D. Wu, C.P. Jiang, H.S. Zhu, H.Y. Jiang, B.J. Cheng and S.W. Zhu
The ZmDULL1 gene encodes a starch synthase and is a determinant of the structure of endosperm starch in maize (Zea mays L.). However, little is known regarding the regulatory mechanism of the ZmDULL1 gene. In this study, we isolated and characterized the ZmDULL1 promoter (PDULL1), which is the 5' flanking region of ZmDULL1 in maize. Sequence analysis show.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.17
- DOI:
- 10.4238/2015.May.22.17