Authors: V.B. Pedrosa, J.P. Eler, J.B.S. Ferraz and E. Groeneveld
Data from 69,525 animals were used to compare two types of analyses, one of them having the weaning management group (WEMANG) included as an effect in the contemporary group (F_WEMANG) and the other considering the weaning management group as a random effect, not related to the mathematical model (R_WEMANG) for post-weaning tr.. Read More»
Authors: Euglena gracilis; Sodium dodecyl sulfate-Tris;RNA isolation; Reverse transcription-polymerase chain reaction
RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit .. Read More»
Authors: A.L. Ferreira Jr, R. Maggioni, D. Concei�§�£o, L.M. Perazzolo and R.L. Petersen
Single nucleotide polymorphisms (SNPs) are the best genetic markers for associative studies of the immune system in invertebrates. In the marine shrimp Litopenaeus vannamei, SNPs linked to disease resistance have been reported for some genes, such as hemocyanin, anti-lipopolysaccharide factor, and heat-shock protein 70 (Hsp70).. Read More»
Authors: F. PÃ?©rtille, R. Zanella, A.M. FelÃ?Âcio, M.C. Ledur, J.O. Peixoto and L.L. Coutinho
Genetic selection for production traits has resulted in a rapid improvement in animal performance and development. Previous studies have mapped quantitative trait loci for body weight at 35 and 41 days, and drum and thigh yield, onto chicken chromosome 4. We investigated this region for single nucleotide polymorphisms and their associations with important.. Read More»
Authors: T. Kumchoo and S. Mekchay
The nuclear receptor subfamily 4, group A, member 1 (NR4A1) and guanine nucleotide binding protein beta polypeptide 2 like-1 (GNB2L1) genes are expressed during the ovulatory process and in early pregnancy in pigs. The objective of this study was to analyze the effects of NR4A1 and GNB2L1 gene variants on reproductive traits in commercial pig breeds. Two .. Read More»
Authors: D.M. Naim, S. Telfer, S. Tatman, S. Bird, S.J. Kemp and P.C. Watts
Movement is an important life history trait that can have an impact on local adaptation, and other evolutionary phenomena. We used a combination of nestbox survey data and genetic techniques (genotyping at 10 microsatellite loci) to quantify patterns of movement in common dormice Muscardinus avellanarius at two distinct sites .. Read More»
Authors: N.B. Stafuzza, M.M. Borges and M.E.J. Amaral-Trusty
In this study, we compared the complete sequence of the FABP6 gene from an animal representing the Murrah breed of the river buffalo (Bubalus bubalis) with the gene sequence from different mammals. The buffalo FABP6 gene is 6105 bp in length and is organized into four exons (67, 176, 90, and 54 bp), three introns (1167, 1737, and 2649 bp), a 5êÂ?Â?UTR (9.. Read More»
Authors: F. Chen, J. Zhou, Y. Xue, S. Yang, M. Xiong, Y. Liand Q. Liu
A single nucleotide polymorphism (SNP) in the TNRC9 gene was identified as a breast cancer susceptibility genetic variant in recent genome-wide association studies of women of European ancestry. We investigated whether TNRC9 polymorphisms are associated with risk of breast cancer in Chinese women of the Han nationality. We gen.. Read More»
Authors: Z.Q. Yuan, J.Y. Zhang and B.S. Zhao
We examined STAG-related gene (DjStag) expression in the planarian Dugesia japonica. This species is common in Far Eastern countries. The DjStag cDNA includes 1362 bp and contains a 489-bp open reading frame corresponding to a deduced protein of 162 amino acids, with a 170-bp 5'-UTR and a 703-bp 3'-UTR. Phylogenetic analysis s.. Read More»
Authors: Y.M. Li, S. Si, P.C. Guo, L.L. Li, C.Y. Bai and S.Q. Yan
The quantity, quality, and distribution of eumelanin and pheomelanin determine a wide variety of coat colors in animals. Three coat color variants exist in farmed wild-type Chinese raccoon dog (Nyctereutes procyonoides procyonoides), which is an important fur-bearing animal species. The ASIP gene is an important candidate gene for coat color variation in .. Read More»