Animal Genetics
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
Authors: N.M.A. Rahman, H. Fu, H. Qiao, S. Jin, H. Bai, W. Zhang, F.W. Jiang, G. Liang, S. Sun, Y. Gong, F.F. Jiang, Y. Xiong and Y. Wu
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different deve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027950
- DOI:
- 10.4238/gmr.15027950
Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae
Authors: C.M. Li, B.M. Fu, L.C. Zhang, B. Tang, L. Zhu, Y. Zhao and J. Zhang
Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028178
- DOI:
- 10.4238/gmr.15028178
Polymorphisms and alternative splicing of the luteinizing hormone receptor of dairy cattle
Authors: S. Wohlres-Viana, E.K.N. Arashiro, D.R.L. Reis, L.E. Fernandes, M.G.C.D. Peixoto, M.A. Machado and J.H.M. Viana
The aim of this study was to screen for variability in the luteinizing hormone/choriogonadotropin receptor (LHCGR) and to determine the occurrence of LHCGR mRNA isoforms in two dairy breeds of cattle. Granulosa cells from dominant ovarian follicles were recovered from 16 Gir and 16 Holstein cows, and total RNA was extracted. C.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027046
- DOI:
- 10.4238/gmr.15027046
Artesunate modulates expression of matrix metalloproteinases and their inhibitors as well as collagen-IV to attenuate pulmonary fibrosis in rats
Authors: Y. Wang, G. Huang, B. Mo and C. Wang
The aim of this study was to determine the effect of artesunate on extracellular matrix (ECM) accumulation and the expression of collagen-IV, matrix metalloproteinase (MMP), and tissue inhibitor of matrix metalloproteinase (TIMP) to understand the pharmacological role of artesunate in pulmonary fibrosis. Eighty Sprague-Dawley .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027530
- DOI:
- 10.4238/gmr.15027530
Hepatic phosphoenolpyruvate carboxykinase expression after gastric bypass surgery in rats with type 2 diabetes mellitus
Authors: J. Wu, S.S. Hou, W. Wang, M. Yin, N. Cheng, L.L. Ge, J.J. Yin and J. Xu
The objective of this study was to investigate the mRNA expression of hepatic phosphoenolpyruvate carboxykinase (PEPCK) after gastric bypass surgery (GBS) in rats with type 2 diabetic mellitus (T2DM). Thirty-six male Goto-Kakizaki rats, aged 12 weeks, were randomly divided into the GBS, sham operation with diet restriction (SO), and sham operation alone (.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.22
- DOI:
- 10.4238/2015.December.14.22
Selected representative microsatellite loci for genetic monitoring and population structure analysis of miniature swine
Authors: C. Wang,L.L. Xu,Y.H. Wu,X.Y. Du,X.Y. Huo,J. Gao,Z.K. Li,Y.C. Li and Z.W. Chen
To optimize the combination of microsatellite loci for genetic control of outbred swine stocks, 32 of 100 loci distributed among almost all chromosomes (except 12) were screened out by 1.5% agarose, 8% polyacrylamide gel and capillary electrophoresis scanning among 3 miniature swine outbred stocks, namely Bama (BM), Guizhou (GZ) and Tibeta (TB). The mean .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.5
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.5
Effect and mechanism of different ways of transplanting bone marrow mesenchymal stem cells in cardiopulmonary resuscitation in rats
Authors: L.L. Zhou, J.K. Liang, Q.M. Lin and Z.T. Huang
The effect and mechanism of different ways of transplanting bone marrow mesenchymal stem cells (MSCs) were explored for treating cardiopulmonary resuscitation in a rat model. Rats were divided into the cardiopulmonary resuscitation group (Group Con), the stereotactic lateral ventricle transplantation group (Group LV), the inte.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.7
- DOI:
- 10.4238/2014.September.29.7
Expression profiles of retinoic acid synthetases ALDH1As and metabolic enzymes CYP26s in adult and embryonic zebrafish (Danio rerio)
Authors: J. Xi,J. Yue and Z. Yang
Retinoic acid (RA) plays a crucial role in cellular proliferation, differentiation, and apoptosis. The physiological activity of RA begins early in development and continues throughout an organism’s life. RA distribution is tightly controlled by the RA synthetases ALDH1As and the metabolic enzymes CYP26s. We analyzed the expressions of ALDH1As and C.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.9
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.9
Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome
Authors: E.R. Monteiro, D.K. Strioto, A.C.S. Meirelles, C.A. Mangolin and M.F.P.S. Machado
Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.2
- DOI:
- 10.4238/2015.December.15.2
Development of novel microsatellite markers for conservation genetic studies of Vulpes vulpes (Canidae) by using next-generation sequencing method
Authors: J.-N. Yu,C.-U. Chung,K.H. Oh,B.-K. Lee and C.E. Lim
The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles) .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.12