Animal Genetics
Inverted migration of rare whisker sheatfish in Nong-Han Lake, northeastern Thailand: Implications for conservation
Authors: P. Phongkaew, U. Arunyawat, A. Swatdipong and V. Hongtrakul
Nong-Han Lake, Thailand, sustains the whisker sheatfish (Micronema bleekeri Günther, 1864), which is a rare species of freshwater catfish. Wild-caught whisker sheatfish has been intensively harvested to meet market demand; yet, genetic information about this species remains unknown. To assist with the in situ.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.16
- DOI:
- 10.4238/2014.September.12.16
Estimating additive and dominance variances for complex traits in pigs combining genomic and pedigree information
Authors: E.V. Costa, D.B. Diniz, R. Veroneze, M.D.V. Resende, C.F. Azevedo, S.E.F. Guimaraes, F.F. Silva and P.S. Lopes
Knowledge of dominance effects should improve genetic evaluations, provide the accurate selection of purebred animals, and enable better breeding strategies, including the exploitation of heterosis in crossbreeds. In this study, we combined genomic and pedigree data to study the relative importance of additive and dominance genetic variation in growth and.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.4
- DOI:
- 10.4238/2015.June.11.4
DKK1 eukaryotic expression plasmid and expression product identification
Authors: G.Y. Bao, K.Y. Lu, S.F. Cui and L. Xu
We constructed the human dickkopf 1 (DKK1) eukaryotic expression plasmid and expressed, purified, and identified its expression product. We extracted cancer cells from cervical cancer tissue, followed by extraction of mRNA. Reverse transcription-polymerase chain reaction was conducted to obtain DKK1 gene fragments. Using these fragments, we prepared the r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.5
- DOI:
- 10.4238/2015.June.11.5
Molecular characterization of the gene checkpoint homolog 1 in Daphnia carinata during different reproductive phases
Authors: L. Kong, H.X. Li, D.L. Wu, G.R. Xu, D.L. Wang and Y.L. Zhao
Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026132
- DOI:
- 10.4238/gmr.15026132
Development of polymorphic microsatellite loci for a new fish species, Chinese sillago (Sillago sinica)
Authors: N. Song, L.Y. Wang, L.N. Yin, Z.Q. Han and T.X. Gao
Sillago sinica is a newly identified species belonging to Sillaginidae, Perciforms, and was found along the coast of China in 2011. In the present study, 81 microsatellite loci were isolated from an enriched genomic library, and 24 positive clones containing microsatellite repeats had adequate flanking sequences for the develo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027212
- DOI:
- 10.4238/gmr.15027212
Polymorphism in the A2M gene associated with high-quality milk in Murrah buffaloes (Bubalus bubalis)
Authors: A.C. Freitas, G.M.F. de Camargo, R.R. Aspilcueta-Borquis, N.B. Stafuzza, G.C. Venturini, F. Tanamati, N.A. Hurtado-Lugo, C.C. Barros and H. Tonhati
The study of genes associated with host defense mechanisms, such as the A2M gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. The aim of this study was to identify polymorphisms in the A2M gene in Murrah buffaloes (Bubalus bubalis), and investigate their associations with milk yiel.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027637
- DOI:
- 10.4238/gmr.15027637
Comparison of EGFR mutation rates in lung adenocarcinoma tissue and pleural effusion samples
Authors: Y. Guan, Z.J. Wang, L.Q. Wang, D.F. Hua and J. Liu
The goal of the current study was to investigate the differences in epidermal growth factor receptor (EGFR) mutation rates in tumor tissue and pleural effusion specimens from patients with lung adenocarcinoma. PCR amplification and gene sequencing were used to detect EGFR mutations in exons 18, 19, 20, and 21 in tumor tissue a.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027001
- DOI:
- 10.4238/gmr.15027001
Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression
Authors: W. Gao, W. Sun, R. Su, X.Y. Lv, Q.Z. Wang, D. Li, H.H. Musa, L. Chen, H. Zhou, H.S. Xu and W.H. Hua
RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027260
- DOI:
- 10.4238/gmr.15027260
Co-expression network analysis prioritizes signaling pathways regulating liver regeneration after partial hepatectomy in rats
Authors: Y. Zhou, J.C. Xu and C.S. Xu
The liver has extraordinary powers of regeneration following partial hepatectomy (PH). Changes in gene expression levels play a key role in cell proliferation and differentiation during liver regeneration (LR). To understand the molecular mechanisms underlying LR, this study was designed to assess the time-dependent changes in.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027596
- DOI:
- 10.4238/gmr.15027596
Effect of inclusion or non-inclusion of short lactations and cow and/or dam genetic group on genetic evaluation of Girolando dairy cattle
Authors: A.W. Canaza-Cayo, M.V.G.B. Silva, J.A. Cobuci, M.F. Martins and P.S. Lopes
The objective of this study was to evaluate the effects of inclusion or non-inclusion of short lactations and cow (CGG) and/or dam (DGG) genetic group on the genetic evaluation of 305-day milk yield (MY305), age at first calving (AFC), and first calving interval (FCI) of Girolando cows. Covariance components were estimated by .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027768
- DOI:
- 10.4238/gmr.15027768