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Triticum Aestivum

Plant Genetics   Research Article

Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi

Authors: P.R. Da-Silva S.P. Brammer D. Guerra S.C.K. Milach A.L. Barcellos M.I. Baggio

Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian whea.. Read More»

Genet. Mol. Res. 11(3):
2012.August.24.7
DOI:
10.4238/2012.August.24.7
Plant Genetics   Research Article

Molecular characterization of wheat germplasm using microsatellite markers

Authors: S. Ijaz and I.A. Khan

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into di.. Read More»

Genet. Mol. Res. 8(3):
Plant Genetics   Research Article

Variation in genotypic responses and biochemical analysis of callus induction in cultivated wheat

Authors: M.M. Shah1, Q. Khalid1, U.W. Khan1, S.A.H. Shah3, S.H. Shah2,A. Hassan1 and A. Pervez1

Wheat is notorious for callus induction, which is a major hindrance in direct gene transfer and consequently for genetic improvement programs. In order to provide a successful platform for gene transformation, good callus quantity and quality is impor­tant. We investigated the variation in callus induction capabilities of Pakistani wheat cultivars and.. Read More»

Genet. Mol. Res. 8(3):
vol8-3gmr513
DOI:
10.4238/vol8-3gmr513
Human Genetics   Research Article

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

Authors: Z.J. Yang, Z.S. Peng and H. Yang

Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potent.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017509
DOI:
10.4238/gmr.15017509
Plant Genetics   Research Article

Identification and evaluation of endogenous control genes for use in quantitative RT-PCR during wheat (Triticum aestivum L.) grain filling

Authors: D. Wu, J. Dong, Y.J. Yao, W.C. Zhao and X. Gao

The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, N.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.8.15
DOI:
http://dx.doi.org/10.4238/2015.September.8.15
Plant Genetics   Research Article

Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA

Authors: Iqbal A, Khan AS, Khan IA, Awan FS, Ahmad A and Khan AA

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polym.. Read More»

Genet. Mol. Res. 6(3):
Plant Genetics   Research Article

Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan

Authors: F. Yasmeen, H. Khurshid and A. Ghafoor

Wheat flour quality is an important consideration in the breeding and development of new cultivars. A strong association between high-molecular weight glutenin subunits (HMW-GS) and bread making quality has resulted in the widespread utilization of HMW-GS in wheat breeding. In this study, we analyzed 242 lines of wheat, including landraces from the provin.. Read More»

Genet. Mol. Res. 14(2):
2015.May.11.15
DOI:
10.4238/2015.May.11.15
Plant Genetics   Research Article

Characterization of agronomic and quality traits and HSW-G5 compositions from the progenies of common wheat (Triticum aestivum L.) with different protein content

Authors: M. Bian, D.K. Sun, D.F. Sun and G.L.Sun

High molecular weight glutenin subunits (HMW-GS) play an essential role in wheat processing quality. In this study, we evaluated the genetic pattern with HMW-GS composition between generations and examined whether agronomic and quality traits were correlated with each other. A wheat (Triticum aestivum L.) cultivar with high protein content and 2 cultivars.. Read More»

Genet. Mol. Res. 14(1):
2015.March.20.7
DOI:
10.4238/2015.March.20.7
Plant Genetics   Research Article

Genetic progress in homogeneous regions of wheat cultivation in Rio Grande do Sul State, Brazil

Authors: D.N. Follmann, A. Cargnelutti Filho, A.D. LĂ?Âșcio, V.Q. de Souza, M. Caraffa, C.A. Wartha

The State of Rio Grande do Sul (RS) stands out as the largest wheat producer in Brazil. Wheat is the most emphasized winter cereal in RS, attracting public and private investments directed to wheat genetic breeding. The study of genetic progress should be performed routinely at breeding programs to study the behavior of cultiv.. Read More»

Genet. Mol. Res. 16(1):
gmr16019517
DOI:
10.4238/gmr16019517
Human Genetics   Research Article

Identification of miRNAs and their targets in wheat (Triticum aestivum L.) by EST analysis

Authors: J. Han, M.L. Kong, H. Xie, Q.P. Sun, Z.J. Nan, Q.Z. Zhang and J.B. Pan

MicroRNAs (miRNAs) are a newly discovered class of noncoding small RNAs that regulate gene expression by directing target mRNA cleavage or translational inhibition. A large number of miRNAs have been identified in plants. Increasing evidence has shown that miRNAs play multiple roles in plant biological processes. So far, identification of miRNAs has been .. Read More»

Genet. Mol. Res. 12(3):
2013.September.19.11
DOI:
10.4238/2013.September.19.11