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Species Identification

Plant Genetics   Research Article

Development of primer pairs from diverse chloroplast genomes for use in plant phylogenetic research

Authors: Y.C. Yang, T.L. Kung, C.Y. Hu and S.F. Lin

Variation in the chloroplast DNA sequence is useful for plant phylogenetic studies. However, the number of variable sequences provided by chloroplast DNA for suggested genes or genomic regions in plant phylogenetic analyses is often inadequate. To identify conserved regions that can be used to design primers and amplify variab.. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.51
DOI:
10.4238/2015.November.18.51
Animal Genetics   Research Article

Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI

Authors: L.H.O. Falc�£o, A.S. Campos, J.E.P. Freitas, M.A.A. Furtado-Neto and V.V. Faria

Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for.. Read More»

Genet. Mol. Res. 16(2):
gmr16029607
DOI:
10.4238/gmr16029607
Microbial Genetics   Research Article

Morphometric and genetic changes in a population of Apis mellifera after 34 years of Africanization

Authors: T.M. Francoy, D. Wittmann, V. Steinhage, M. Drauschke, S. M�¼ller, D.R. Cunha, A.M. Nascimento, V.L.C. Figueiredo, Z.L.P. Sim�µes, D. De Jong, M.C. Arias and L.S. Gon�§alves

Though the replacement of European bees by Africanized honey bees in tropical America has attracted considerable attention, little is known about the temporal changes in morphological and genetic characteristics in these bee populations. We examined the changes in the morphometric and genetic profiles of an Africanized honey b.. Read More»

Genet. Mol. Res. 8(2):
Plant Genetics   Research Article

Identification of Uvaria sp by barcoding coupled with high-resolution melting analysis (Bar-HRM)

Authors: M. Osathanunkul1, P. Madesis2, S. Ounjai1, K. Pumiputavon1, R. Somboonchai3, P. Lithanatudom1, T. Chaowasku1, J. Wipasa4 and C. Suwannapoom5

DNA barcoding, which was developed about a decade ago, relies on short, standardized regions of the genome to identify plant and animal species. This method can be used to not only identify known species but also to discover novel ones. Numerous sequences are stored in online databases worldwide. One of the ways to save cost and time (by omitting the sequ.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017405
DOI:
10.4238/gmr.15017405
Plant Genetics   Research Article

Gender identification of five genera of stingless bees (Apidae, Meliponini) based on wing morphology

Authors: T.M. Francoy R.A.O. Silva P. Nunes-Silva C. Menezes V.L. Imperatriz-Fonseca

Currently, the identification of pollinators is a critical necessity of conservation programs. After it was found that features extracted from patterns of wing venation are sufficient to discriminate among insect species, various studies have focused on this structure. We examined wing venation patterns of males and workers of.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr557
DOI:
10.4238/vol8-1gmr557
Animal Genetics   Research Article

Utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species.

Authors: J.-J. Wilson1,2*, K.-W. Sing1*, M.R.A. Halim2, R. Ramli2,R. Hashim2 and M. Sofian-Azirun2

Bats are important flagship species for biodiversity research; however, diversity in Southeast Asia is considerably underestimated in the current checklists and field guides. Incorporation of DNA barcoding into surveys has revealed numerous species-level taxa overlooked by conventional methods. Inclusion of these taxa in inven.. Read More»

Genet. Mol. Res. 13(1):
2014.February.19.2
DOI:
10.4238/2014.February.19.2
Plant Genetics   Research Article

DNA barcoding for efficient identification of Ixiolirion species (Ixioliriaceae)

Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang

Ixiolirion is a genus of unresolved taxonomy. DNA barcoding is a technique that allows species identification using standardized DNA sequences. In this study, a total of 23 individuals, representing 2 Chinese Ixiolirion species, were sampled to test the effectiveness of 3 DNA barcodes [internal transcribed spacer (ITS), chlorop.. Read More»

Genet. Mol. Res. 14(1):
2015.March.13.19
DOI:
10.4238/2015.March.13.19
Human Genetics   Research Article

Ancient DNA in historical parchments - identifying a procedure for extraction and amplification of genetic material

Authors: T. Lech

Historical parchments in the form of documents, manuscripts, books, or letters, make up a large portion of cultural heritage collections. Their priceless historical value is associated with not only their content, but also the information hidden in the DNA deposited on them. Analyses of ancient DNA (aDNA) retrieved from parchm.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028661
DOI:
10.4238/gmr.15028661
Plant Genetics   Research Article

Rapid discrimination between four seagrass species using hybrid analysis

Authors: M. Osathanunkul,P. Madesis,S. Ounjai,C. Suwannapoom and A. Jampeetong

Biological species are traditionally identified based on their morphological features and the correct identification of species is critical in biological studies. However, some plant types, such as seagrass, are taxonomically problematic and difficult to identify. Furthermore, closely related seagrass species, such as Halophila spp, form a taxonomically u.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.27.10
DOI:
http://dx.doi.org/10.4238/2015.April.27.10
Human Genetics   Research Article

Molecular identification of Achyranthis Bidentatae Radix by using DNA barcoding

Authors: D.-Y. Hou, G.-P. Wang, L.-H. Zhi, H.-W. Xu, H.-L. Liang, M.-M. Yang and G.-A. Shi

Achyranthis Bidentatae Radix has a long history in China as a commonly used herb that can be used to treat various diseases, including those related to the liver, muscles, bones, and kidneys. Recently, an increase in the number of adulterants has been reported, which affects the clinical safety of Achyranthis Bidentatae Radix... Read More»

Genet. Mol. Res. 15(2):