Authors: Hua-Shan Yi and Xianping Ma
Investigate the function of BmICE-2 gene in silkworm cells by constructing a BmICE-2 interference vector and analysing its interference effect. Online RNAi design software was used to predict the interference target sequence of Bmice-2. An optimized sequence was selected and inserted into the backbone of interference microRNA... Read More»
Authors: L.F.C. Ribeiro D.B. Zanatta J.P. Bravo R.M.C. Brancalh�£o M.A. Fernandez
The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance.. Read More»
Authors: R.M.C. Brancalh�£o E.F.B. Torquato M.A. Fernandez
A multiple nucleopolyhedrovirus previously isolated from infected Bombyx mori L. larvae (BmMNPV) in Paraná state, Brazil, was inoculated into B. mori larvae to examine susceptibility and cytopathology in silk gland cells. The anterior, middle and posterior silk glands were removed from the infected silkworm at different.. Read More»
Authors: J.F. Barbosa J.P. Bravo D.B. Zanatta J.L.C. Silva M.A. Fernandez
Conformation-sensitive gel electrophoresis is a useful method for identifying allele polymorphism; it provides co-dominant molecular markers. Using this method, we identified genetic variability in the third intron of the fibroin light chain gene, fib-L, in six Bombyx mori strains. Only Chinese C21A strain did not demonstrate .. Read More»
Authors: G.Q. Wei, L. Yu, C.L. Liu, B.J. Zhu and H.J. Ding
In the silkworm, Bombyx mori, normal markings are mainly controlled by the +P gene, which is located on the second chromosome. Due to a lack of crossing over in females, reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of the +P gene based on an SSR linkage map using silkworm strains P50 and H9, which are normal marking.. Read More»
Authors: Z. Lie, L. Cheng, D. Fang-yin and F. Shou-min
The quantitative trait loci (QTLs) associated with cocoon traits in silkworms were mapped in 44 individuals of a backcross of Dazao females with hybrid F1 males; the hybrid males were from females of inbred C100 strain, which have white cocoons and superior cocoon traits, crossed with males of inbred strain Dazao, which have g.. Read More»
Authors: L.A. Dourado, L.F.C. Ribeiro, R.M.C. Brancalh�£o, J. Tavares, A.R. Borges and M.A. Fernandez
A nuclear polyhedrosis virus isolated from infected Bombyx mori, BmNPV, was used to inoculate silkworms to determine salivary gland cell susceptibility. The salivary gland was removed from infected silkworms at different times post-inoculation and examined by light microscopy. The salivary gland cells did not exhibit any signs.. Read More»
Authors: Guilherme Gomes, Elaine C.M. Silva-Zacarin, Fernando J. Zara, Regina L.M. Silva de Moraes and Fl�¡vio H. Caetano
The cocoon, produced by most holometabolous insects, is built with silk that is usually produced by the larval salivary gland. Although this silk has been widely studied in the Lepidoptera, its composition and macromolecular arrangement remains unknown in the Hymenoptera. The macromolecular array patterns of the silk in the la.. Read More»