Rna
Effect of DNase treatment on RNA extraction from preimplantation murine embryos
Authors: J. Norhazlin, M.N.K. Nor-Ashikin, B.P. Hoh, S.H. Sheikh Abdul Kadir, S. Norita, M. Mohd-Fazirul, W.J. Wan-Hafizah, D. Razif, M.H. Rajikin and B. Abdullah
The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.1
- DOI:
- 10.4238/2015.August.28.1
Whole-transcriptome sequencing of Pinellia ternata using the Illumina platform
Authors: X. Huang, Y. Jing, D.J. Liu, B.Y. Yang, H. Chen and M. Li
Pinelliae rhizoma is the dried tuber of Pinellia ternata (Thunb.) Breit., and has been used for thousands of years as a traditional Chinese medicine. However, its genomic background is little known. With the development of high-throughput genomic sequencing, it is now easy and cheap to obtain genomic information. In this study.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028062
- DOI:
- 10.4238/gmr.15028062
Phylogenetic relationships of Pseudohynobius (Urodela, Hynobiidae) inferred from DNA barcoding analysis
Authors: Y.-Y. Zhao, L.-N. Su, Z.-M. Zhang and X.-Y. Wang
As a proven tool, DNA barcoding can identify species rapidly and unambiguously. In this study, we used mtDNA cyt b, COI, and 16s rRNA sequences of six species of Pseudohynobius, Protohynobius puxiongensis, Liua shihi, Ranodon sibiricus, and Pachyhynobius shangchengensis, to reconstruct the phylogenetic relationships using Baye.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028155
- DOI:
- 10.4238/gmr.15028155
Genetic effect of an A/G polymorphism in the HSP70 gene on thermotolerance in chicken
Authors: Z.Y. Chen, W.W. Zhang, J.K. Gan, L.N. Kong, X.Q. Zhang,D.X. Zhang and Q.B. Luo
Studying thermotolerance is important for the prevention of thermostress in chickens. This study aimed to analyze the effect of mutations in the heat shock protein 70 (HSP70) gene on chicken thermotolerance. The C.-69A>G SNP in the 5'-flanking region of the HSP70 gene was genotyped in Lingshan and White Recessive Rock (WRR).. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028271
- DOI:
- 10.4238/gmr.15028271
A comprehensive review of microRNA-related polymorphisms in gastric cancer
Authors: B.W. Han Z.H. Li, S.F. Liu, H.B. Han, S.J. Dong, H.J. Zou,R.F. Sun and J. Jia
MicroRNAs (miRNAs) are a class of small non-coding RNA molecules of about 22 nucleotides in length. miRNAs are highly conserved in both plants and animals, and function as gene regulators by binding to the 3'-untranslated region of target mRNAs for cleavage and/or translational repression. miRNA biogenesis, stability, and regu.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028289
- DOI:
- 10.4238/gmr.15028289
MicroRNA screening and functional study of obliterative bronchiolitis in a rat model simulating lung transplantation
Authors: J. Wang, H. Cao, X. Hong, G.H. Chen, H.M. Fan, Q.C. Li, Z.M. Liu and K.F. Li
The aim of this study was to provide the experimental basis for effective prevention and treatment of obliterative bronchiolitis (OB) by studying the changes on the microRNA (miRNA) expression profile after an orthotopic tracheal transplantation (OTT) simulating lung transplantation (LT). The OTT was performed on inbred rats to establish an OB animal mode.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.29.40
- DOI:
- 10.4238/2015.December.29.40
An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Authors: R.R. Oliveira, A.J.C. Viana, A.C.E. Re�¡tegui and M.G.A. Vincentz
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA cytosine methylation, which is an epigenetic mark. Correlation of gene expression with DNA sequence or.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.32
- DOI:
- 10.4238/2015.December.28.32
A bioinformatics analysis of alternative exon usage in human genes coding for extracellular matrix proteins
Authors: Noboru Jo Sakabe, Maria Dulcetti Vibranovski and Sandro Jos�© de Souza
Alternative splicing increases protein diversity through the generation of different mRNA molecules from the same gene. Although alternative splicing seems to be a widespread phenomenon in the human transcriptome, it is possible that different subgroups of genes present different patterns, related to their biological roles. An.. Read More»
- Genet. Mol. Res. 3(4):
Splicing factors are differentially expressed in tumors
Authors: Natanja Kirschbaum-Slager, Graziela M.P. Lopes, Pedro A.F. Galante, Gregory J. Riggins and Sandro J. de Souza
Although alternative splicing of many genes has been found associated with different stages of tumorigenesis and splicing variants have been characterized as tumor markers, it is still not known whether these examples are sporadic or whether there is a broader association between the two phenomena. In this report we evaluated,.. Read More»
- Genet. Mol. Res. 3(4):
Knockdown of chimeric glucoce rebrosidase by green fluorescent protein-directed small interfering RNA
Authors: Tessa N. Campbell, Francis Y.M. Choy
Gaucher disease, the most common type of lysosomal storage disorder, is characterized by an inherited deficiency of the membrane-associated hydrolase, glucocerebrosidase. Glucocerebrosidase catalyzes the hydrolysis of glucocerebroside to ceramide and glucose, a crucial step in the recycling of membrane sphingolipids. The exorb.. Read More»
- Genet. Mol. Res. 3(2):