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Animal Genetics   Research Article

Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets

Authors: W.Y. Qin, L.N. Gan, R.W. Xia, W.H. Dong, S.Y. Sun, G.Q. Zhu,S.L. Wu and W.B. Bao

Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq res.. Read More»

Genet. Mol. Res. 16(2):
gmr16029574
DOI:
10.4238/gmr16029574
Plant Genetics   Research Article

TaALMT1 promoter sequence compositions, acid tolerance, and Al tolerance in wheat cultivars and landraces from Sichuan in China

Authors: C. Han1*, S.F. Dai1*, D.C. Liu1, Z.J. Pu2, Y.M. Wei1, Y.L. Zheng1, D.J. Wen1, L. Zhao1 and Z.H. Yan1

Previous genetic studies on wheat from various sources have indicated that aluminum (Al) tolerance may have originated independently in USA, Brazil, and China. Here, TaALMT1 promoter sequences of 92 landraces and cultivars from Sichuan, China, were sequenced. Five promoter types (I', II, III, IV, and V) were observed in 39 cultivars, and only three promot.. Read More»

Genet. Mol. Res. 12(4):
2013.November.18.9
DOI:
10.4238/2013.November.18.9
Human Genetics   Research Article

Influence of the human MIF promoter polymorphism on hepatocellular carcinoma prognosis

Authors: T. Yuan, C. Tang, M. Chen, S. Deng and P. Chen

Hepatocellular carcinoma (HCC) is one of the most common worldwide malignancies. A relative complete diagnosis system for primary carcinoma of liver has already been established, but the surgical prognosis for HCC, which depends mainly on postoperative pathological classification and data of recurrence and metastasis, lacks valid experimental indicators. .. Read More»

Genet. Mol. Res. 12(4):
2013.January.4.3
DOI:
10.4238/2013.January.4.3
Microbial Genetics   Research Article

Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows

Authors: X.S. Wang, Y. Zhang, Y.H. He, P.P. Ma, L.J. Fan, Y.C. Wang,Y.I. Zhang, D.X. Sun, S.L. Zhang, C.D. Wang, J.Z. Song and Y. Yu

Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyr.. Read More»

Genet. Mol. Res. 12(4):
2013.December.4.10
DOI:
10.4238/2013.December.4.10
Animal Genetics   Research Article

Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter

Authors: P. Lu, X. Feng, J.S. Zhou, X.B. Liu, Q.X. Chen and M. Li

Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ov.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.June.9.19
DOI:
http://dx.doi.org/10.4238/2014.June.9.19
Plant Genetics   Research Article

Cloning and functional analysis of the chitinase gene promoter in peanut

Authors: X.L. Chen, R.T. Song, M.Y. Yu, J.M. Sui, J.S. Wang and L.X. Qiao

Chitinase is an important pathogenesis-related protein in plants, and it can accumulate when induced by salicylic acid (SA) or other elicitors. Here, we found that chitinase mRNA levels were 4.5-times greater when peanut seedlings were sprayed with 1.5 mM SA, as compared to water. The upstream promoter sequence of the chitinas.. Read More»

Genet. Mol. Res. 14(4):
2015.October.19.15
DOI:
10.4238/2015.October.19.15
Cytogenomics   Research Article

Construction of the intermediate vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221

Authors: S.S. Ahmed Z.-H. Gong J.-J. Ji Y.-X. Yin H.-J. Xiao M.A. Khan A. Rehman I. Ahmad

Molecular chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly expanded multiple cloning site with an adjacent imported CaMV35S promoter sequence. This vector allows controlled tr.. Read More»

Genet. Mol. Res. 11(3):
2012.August.31.7
DOI:
10.4238/2012.August.31.7
Bioinformatics   Research Article

Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba

Authors: F. Xu X.H. Huang L.L. Li G. Deng H. Cheng X.F. Rong J.B. Li S.Y. Cheng

Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The .. Read More»

Genet. Mol. Res. 12(1):
2013.February.4.3
DOI:
10.4238/2013.February.4.3
Cytogenomics   Research Article

Characterization of a rice germin-like protein gene promoter

Authors: T. Mahmood T. Yasmin M.I. Haque S.M.S. Naqvi

Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by an.. Read More»

Genet. Mol. Res. 12(1):
2013.February.7.6
DOI:
10.4238/2013.February.7.6
Plant Genetics   Research Article

Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.)

Authors: M. Li1*, S.Z. Zhao1*, C.Z. Zhao1, Y. Zhang1, H. Xia1, J. Lopez-Baltazar2, S.B. Wan1 and X.J. Wang1

SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and anal.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017344
DOI:
10.4238/gmr.15017344

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