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Piglet

Animal Genetics   Research Article

Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets

Authors: W.Y. Qin, L.N. Gan, R.W. Xia, W.H. Dong, S.Y. Sun, G.Q. Zhu,S.L. Wu and W.B. Bao

Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq res.. Read More»

Genet. Mol. Res. 16(2):
gmr16029574
DOI:
10.4238/gmr16029574
Animal Genetics   Research Article

Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig

Authors: X.Y. Huang, Q.L. Yang, J.H. Yuan and S.B. Gun

In this study, 290 Chinese native Yantai black pig piglets were investigated to identify gene polymorphisms, for haplotype reconstruction, and to determine the association between piglet diarrhea and swine leukocyte antigen (SLA) class II DQA exons 2, 3, and 4 by polymerase chain reaction-single stranded conformational polymorphism and cloning sequencing... Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.8.7
DOI:
http://dx.doi.org/10.4238/2015.September.8.7
Animal Genetics   Research Article

Genetic association of sequence variation in exon 3 of the swine leukocyte antigen-DQA gene with piglet diarrhea in Large White, Landrace, and Duroc piglets

Authors: Q.L. Yang, X.Y. Huang, J.J. Kong, S.G. Zhao, L.X. Liu and S.B. Gun

Piglet diarrhea is one of the primary factors that affects the benefits of the swine industry. Recent studies have shown that exon 2 of the swine leukocyte antigen-DQA gene is associated with piglet resistance to diarrhea; however, the contributions of additional exon coding regions of this gene remain unclear. Here, we detected and sequenced variants in .. Read More»

Genet. Mol. Res. 15(3):
gmr.15038673
DOI:
10.4238/gmr.15038673
Microbial Genetics   Research Article

Cloning and expression of the porcine attaching and effacing-associated (paa) gene of enteropathogenic Escherichia coli

Authors: D.A. Pereira, E.C. Teixeira Florian, M.A. Ono, C. Nachi Rossi, O. Vidotto and M.C. Vidotto

Porcine enteropathogenic Escherichia coli (PEPEC) produce an outer membrane protein (intimin) called Paa (porcine attaching and effacing-associated), which is involved in the pathogenesis of E. coli in piglets with diarrhea. The paa gene of a PEPEC strain isolated in Paraná, Brazil, was amplified by polymerase chain reaction, sequenced, and cloned .. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.5
DOI:
10.4238/2015.July.31.5
Microbial Genetics   Research Article

VP6 gene diversity in Brazilian strains of porcine group C rotavirus

Authors: K.C. M�©dici, A.F. Barry, A.F. Alfieri and A.A. Alfieri

Group C rotavirus (RV-C) has been found in Brazilian pig herds; however, wild-type strains have not yet been characterized. We made a molecular analysis of a region of gene 5 in Brazilian RV-C strains. Stool samples from 11 piglets (diarrheic and with normal consistency) positive for the RV-C VP6 gene in an RT-PCR assay were s.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr715
DOI:
10.4238/vol9-1gmr715
Human Genetics   Research Article

Effect of weaning age on cortisol release in piglets

Authors: L.A. Li, J.J. Yang, Y. Li, L. Lv, J.J. Xie, G.M. Du, T.M. Jin, S.Y. Qin and X.L. Jiao

The effect of weaning age on the adrenal cortex, which plays a vital role in the stress response, is currently unknown. Therefore, plasma adrenocorticotropic hormone (ACTH) and cortisol levels, weights and relative weights of adrenal glands, and steroidogenesis-related protein and enzyme expression levels in piglets weaned on .. Read More»

Genet. Mol. Res. 15(2):
gmr.15027693
DOI:
10.4238/gmr.15027693