Odontobutis Potamophila
Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila
Authors: P.P. Wang, M. Ren, S.Q. Chen, S.W. Yin, C. Zhao, H.Y. Zhang, X.R. Li, Q.Q. Cao and G.Q. Zhou
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequen.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029129
- DOI:
- 10.4238/gmr16029129
Development and validation of single nucleotide polymorphism markers in Odontobutis potamophila from transcriptomic sequencing
Authors: H.W. Zhang, S.W. Yin, L.J. Zhang, X.Y. Hou, Y.Y. Wang and G.S. Zhang
Transcriptome sequencing technology has been applied in the development and discovery of single nucleotide polymorphism (SNP) markers in fish. In this study, a panel of 120 expressed sequence tag (EST)-derived SNPs was selected by several selection filters from the resultant EST library of Odontobutis potamophila using Illumina Sequencing. In total, 37 SN.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.18
- DOI:
- 10.4238/2015.March.20.18
Novel polymorphic microsatellite markers in Odontobutis potamophila
Authors: Y.B. Shen, D. Li, J.L. Li, R.Q. Wang and Y.F. Xuan
We characterized 16 novel polymorphic loci isolated from a partial genomic DNA library of Odontobutis potamophila enriched for CA repeats. We tested the variability of these microsatellites on 51 unrelated individuals collected in China. All loci were polymorphic. The average allele number was 14.6 per locus, ranging from 2 to 27. The observed heterozygos.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.9.13
- DOI:
- 10.4238/2015.June.9.13