Authors: J.J. Fan, J.J. Bai, D.M. Ma, L.Y. Yu and P. Jiang
Aldolase is a key enzyme involved in glycolysis, gluconeogenesis, and the pentose phosphate pathway. To establish the expression patterns of all three aldolase isozyme genes in different tissues and during early embryogenesis in lower vertebrates, as well as to explore the functional differences between these three isozymes, t.. Read More»
Authors: B.H. Xu, K.J. Chen, Y.B. Yao, Q.L. Liu, T.Y. Xiao, J.M. Su and H.Z. Peng
Here, we characterized the structure and function of the coagulation factor II (FII) gene in grass carp and determined its role in coagulation mechanisms. The FII gene EST was obtained using a constructed splenic transcriptome database; the full-length FII gene sequence was obtained by 3' and 5' RACE. The open reading frame (ORF) of FII was cloned and the.. Read More»
Authors: B.H. Xu, L. Zhong, Q.L. Liu, T.Y. Xiao, J.M. Su, K.J. Chen, H.Q. Wang, Y.J. Dai and J. Chen
The aim of the study was to investigate the grass carp hemorrhagic infection pathway and its key-related genes. Grass carp reovirus (GCRV) might cause hemorrhagic disease in grass carps. Healthy grass carp fingerlings (N = 60) were divided into control and infected groups. Fish in the control group were intraperitoneally (ip) .. Read More»
Authors: Y.B. Shen, J.B. Zhang, J.J. Fu, X.Y. Xu, J.L. Li R.Q. Wang and Y.F. Xuan
Aeromonas hydrophila, a widespread bacterium in the aquatic environment, causes hemorrhagic septicemia in fish. In the last decade, the disease has caused mass mortalities and tremendous economic loss in cultured fish. The complement component C7 is a terminal component of complement that interacts in a sequence of polymerizat.. Read More»
Authors: L.Y. Yu, J.J. Bai, J.J. Fan, D.M. Ma, Y.C. Quan and P. Jiang
Total RNA isolated from the brain, muscle, liver, gonad, and intestinal tissues of grass carp was pooled to construct cDNA libraries. Using 454 pyrosequencing, a total of 738,604 high-quality reads were generated from the normalized cDNAs of the pooled individuals. Clustering and assembly of these reads produced a set of 37,086 all-unigene sequences after.. Read More»