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Gene

Plant Genetics   Research Article

Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

Authors: J.X. Juan, X.H. Yu, X.M. Jiang, Z. Gao, Y. Zhang, W. Li,Y.D. Duan and G. Yang

ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitati.. Read More»

Genet. Mol. Res. 14(1):
2015.January.30.1
DOI:
10.4238/2015.January.30.1
Plant Genetics   Research Article

Genetic diversity analysis of fruit characteristics of hawthorn germplasm

Authors: K. Su,Y.S. Guo, G. Wang, Y.H. Zhao, W.X. Dong

One hundred and six accessions of hawthorn intraspecific resources, from the National Germplasm Repository at Shenyang, were subjected to genetic diversity and principal component analysis based on evaluation data of 15 fruit traits. Results showed that the genetic diversity of hawthorn fruit traits varied. Among the 15 traits.. Read More»

Genet. Mol. Res. 14(4):
2015.December.7.14
DOI:
10.4238/2015.December.7.14
Plant Genetics   Research Article

Development of simple sequence repeat markers in persimmon (Diospyros L.) and their potential use in related species

Authors: Y. Yang, Z.B. Jing, X.F. Ruan and J.M. Cheng

Persimmon (Diospyros L.) is an economically important fruit in the world, and it has been recognized as a healthy nutrient supply for human consumption. In this study, 14 microsatellite markers were developed from an AG/TC and AC/TG-enriched genomic library of Chinese persimmon Mopanshi. Twelve polymorphic markers were selected in 4 related species; these.. Read More»

Genet. Mol. Res. 14(1):
2015.January.30.2
DOI:
10.4238/2015.January.30.2
Animal Genetics   Research Article

RNA-Seq-based transcriptome and the reproduction-related genes for the aphid Schlechtendalia chinensis (Hemiptera, Aphididae)

Authors: P. Liu, Z.X. Yang, X.M. Chen, P. Yang

Chinese galls form on sumac plants (Rhus chinensis) and are used for medicinal and chemical purposes, due to their richness in tannins. The galls are formed by aphids, the most prominent of which is Schlechtendalia chinensis, which forms horn-shaped galls on the winged rachis of R. chinensis. S. chinensis has a complex life cy.. Read More»

Genet. Mol. Res. 16(1):
gmr16019448
DOI:
10.4238/gmr16019448
Animal Genetics   Research Article

Polymorphisms in the ovine myostatin gene are associated with birth weight but not with weight gain in Iranian Makoei sheep

Authors: M. Farhadian A. Hashemi K. Mardani R. Darvishzadeh S. Jafari

Myostatin, a transforming growth factor-beta superfamily member, has been well documented as a negative regulator of muscle growth and development. Myostatin, which has 376 amino acids, is synthesized as a precursor protein. Polymorphism of the myostatin gene in Makoei sheep was investigated by PCR and single-strand conformation polymorphism technique (SSCP).. Read More»

Genet. Mol. Res. 11(4):
2012.October.4.4
DOI:
10.4238/2012.October.4.4
Animal Genetics   Research Article

Genetic variation of Sargassum horneri populations detected by inter-simple sequence repeats

Authors: J.R. Ren, R. Yang, Y.Y. He and Q.H. Sun

The seaweed Sargassum horneri is an important brown alga in the marine environment, and it is an important raw material in the alginate industry. Unfortunately, the fixed resource that was originally reported is now reduced or disappeared, and increased floating populations have been reported in recent years. We sampled a floating population and 4 fixed c.. Read More»

Genet. Mol. Res. 14(1):
2015.January.30.3
DOI:
10.4238/2015.January.30.3
Plant Genetics   Research Article

Spittle protein profile of Mahanarva spectabilis (Hemiptera: Cercopidae) fed various elephant grass genotypes

Authors: A.M. Auad M.F. Martins I. Fonseca S.V. Paula-Moraes M.M. Kopp M.C. Cordeiro

An understanding of the interaction between spittlebugs and forage grasses is essential for establishing factors that favor productive pastures. In the present study, we evaluated the protein profiles of the spittle of Mahanarva spectabilis (Distant, 1909) (Hemiptera: Cercopidae) fed various elephant grass genotypes. Each plant was infested with a single fif.. Read More»

Genet. Mol. Res. 11(4):
2012.October.4.7
DOI:
10.4238/2012.October.4.7
Bioinformatics   Research Article

Evolutive and structural characterization of Nostoc commune iron-superoxide dismutase that is fit for modification

Authors: Y. Ma M. Lu J.-Y. Li Y. Qin X.-G. Gong

Superoxide dismutase (SOD) has extensive clinical applications for protecting organisms from toxic oxidation. In this study, the integrated iron-superoxide dismutase gene (fe-sod) coding sequence of Nostoc commune stain CHEN was cloned from genomic DNA and compared to sods from other reported algae. These analyses of immunology and phylogenetics indicated th.. Read More»

Genet. Mol. Res. 11(4):
2012.October.4.8
DOI:
10.4238/2012.October.4.8
Plant Genetics   Research Article

Myrciaria dubia, an Amazonian fruit: population structure and its implications for germplasm conservation and genetic improvement

Authors: C.F. Nunes, T.A. Setotaw, M. Pasqual, E.A. Chagas, E.G. Santos,D.N. Santos, C.G.B. Lima, G.M.A. Can�§ado

Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encou.. Read More»

Genet. Mol. Res. 16(1):
gmr16019409
DOI:
10.4238/gmr16019409
Plant Genetics   Research Article

Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae

Authors: B.B. Cheng, Q.W. Sun,Y.Q. Zheng

Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of.. Read More»

Genet. Mol. Res. 14(4):
2015.December.7.15
DOI:
10.4238/2015.December.7.15