
Authors: D. Wu, J. Dong, Y.J. Yao, W.C. Zhao and X. Gao
The use of appropriate reference genes is essential for the generation of accurate and biologically meaningful results from quantitative real-time PCR (qRT-PCR) analysis. However, studies have found that the expression of most commonly used reference genes is not always independent of the tissues, treatments, or developmental stages studied. geNormPlus, N.. Read More»
Authors: T. Nakamura, N. Okamura, M. Yagi, H. Omatsu, M. Yamamori,A. Kuwahara, K. Nishiguchi, M. Horinouchi, K. Okumura and T. Sakaeda
ABCB1, also known as MDR1/P-glycoprotein, can transport cortisol and aldosterone. We examined the effects of ABCB1 polymorphisms on serum levels of cortisol and aldosterone among different phases of the normal menstrual cycle in 51 non-pregnant healthy Japanese female volunteers (22 ± 1 years old). The menstrual cycle w.. Read More»
Authors: N.K. Korir, W. Diao, R. Tao, X. Li, E. Kayesh, A. Li, W. Zhen and S. Wang
The genetic diversity and relationship of 42 tomato varieties sourced from different geographic regions was examined with EST-SSR markers. The genetic diversity was between 0.18 and 0.77, with a mean of 0.49; the polymorphic information content ranged from 0.17 to 0.74, with a mean of 0.45. This indicates a fairly high degree .. Read More»
Authors: Y. Cheng, Z.P. Yao, M.Y. Ruan, Q.J. Ye, R.Q. Wang, G.Z. Zhou, J. Luo, Z.M. Li, Y.J. Yang and H.J. Wan
The WRKY family is one of the most important transcription factor families in plants, involved in the regulation of a broad range of biological roles. The recent releases of whole-genome sequences of pepper (Capsicum annuum L.) allow us to perform a genome-wide identification and characterization of the WRKY family. In this study, 61 CaWRKY proteins were .. Read More»
Authors: L. Yang, X.W. Wang, L.P. Zhu, H.L. Wang, B. Wang, T. Wu, Q. Zhao, D.L.X.T. JinSiHan and X.Y. Wang
Activity of methylenetetrahydrofolate reductase (MTHFR), an enzyme involved in folate metabolism, is influenced by mutations in the corresponding gene, contributing to a decrease in 5,10-MTHF. Due to such polymorphisms, individuals differ in MTHFR enzyme activity and plasma folate levels. We investigated the relationship between two common MTHFR polymorph.. Read More»
Authors: J.Y. Du, H. Yang, D.R. Tian, Q.M. Wang and L. He
We looked for differentially expressed genes at different stages of preadipocyte differentiation and examined their functions, based on DNA microarrays of preadipocytes obtained from healthy subjects undergoing cosmetic liposuction. We downloaded gene expression profile GSE25910 from the Gene Expression Omnibus database and id.. Read More»
Authors: J.S. Freitas, E.M. Silva, A. Rossi
The present study was designed to identify nutrient-dependent changes in extracellular pH and acid phosphatase secretion in the biA1 palC4 mutant strain of Aspergillus nidulans. The palC4 mutant was selected as lacking alkaline phosphatase, but having substantially increased acid phosphatase activity when grown on solid minima.. Read More»
Authors: Y. Sheu, A.S. Cunha-Machado, A.B.P.L. Gontijo, F.C. Favoreto,T.C.B. Soares and F.D. Miranda
The Bromeliaceae family includes a range of species used for many purposes, including ornamental use and use as food, medicine, feed, and fiber. The state of Espírito Santo, Brazil is a center of diversity for this family in the Atlantic Forest. We evaluated the genetic diversity of five populations of the Bromeliaceae .. Read More»
Authors: A. Cornejo-Romero, J. Medina-S�¡nchez, T. Hern�¡ndez-Hern�¡ndez,B. Rend�³n-Aguilar, P.L. Valverde, A. Zavala-Hurtado,S.P. Rivas-Arancibia, M.A. P�©rez-Hern�¡ndez, G. L�³pez-Ortega,C. Jim�©nez-Sierra and C.F. Vargas-Mendoza
The endemic Mexican cactus, Mammillaria pectinifera, shows low dispersal capabilities and isolated populations within the highly dissected landscape of Tehuacán Valley. These characteristics can restrict gene flow and act upon the genetic divergence and speciation in aridplants. We conducted a phylogeographic study to d.. Read More»
Authors: R.X. Soares, M.B. Cioffi, L.A.C. Bertollo, A.T. Borges, G.W.W.F. Costa and W.F. Molina
Sphyraena (barracudas) represents the only genus of the Sphyraenidae family and includes 27 species distributed into the tropical and subtropical oceanic regions. These pelagic predators can reach large sizes and, thus, attracting significant interest from commercial and sport fishing. Evolutionary data for this fish group, as.. Read More»