Gene
Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells
Authors: Y. Xu,X.J. Zhang,L. Fang and T.B. Zhao
To investigate the effect on cell proliferation and extracellular matrix expression of annulus fibrosus (AF) cells when co-cultured with bone marrow mesenchymal stem cells (BMSCs). Primary isolated rabbit BMSCs and AF cells were cultured and harvested cells were placed into a 15-mL centrifugal tube co-culture system in a ratio of 2:1 (A group), 1:1 (B gro.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.7
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.7
CpG island evolution in the mammalian DHRS4 gene cluster and its role in the regulation of gene transcription
Authors: Z. Su, G. Liu, X. Song, B. Liang, X. Chang and D. Huang
The dehydrogenase/reductase (SDR family) member 4 (DHRS4) gene is copied during mammalian evolution; therefore, while only one DHRS4 gene is expressed in the mouse genome, the gene cluster consists of two (DHRS4 and DHRS4L1) and three (DHRS4, DHRS4L2, and DHRS4L1) copies in chimpanzees and humans, respectively. In this study, .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027752
- DOI:
- 10.4238/gmr.15027752
Genetic divergence between Mexican Opuntia accessions inferred by polymerase chain reaction-restriction fragment length polymorphism analysis
Authors: S. Samah, E. Valadez-Moctezuma, K.S. Pel�¡ez-Luna, S. Morales-Manzano, P. Meza-Carrera and R.C. Cid-Contreras
Molecular methods are powerful tools in characterizing and determining relationships between plants. The aim of this study was to study genetic divergence between 103 accessions of Mexican Opuntia. To accomplish this, polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of three chloroplast interge.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027786
- DOI:
- 10.4238/gmr.15027786
Assessment of genetic diversity of wheat genotypes by resistance gene analog-EST markers
Authors: O. Karakas, F. Gurel and A.A. Uncuoglu
Resistance gene analog-expressed sequence tag (RGA-EST)-based markers have been used for variety discrimination and studies of genetic diversity in wheat. Our aim is to increase the competitiveness of public wheat breeding programs through intensive use of modern selection technologies, mainly marker-assisted selection. The ge.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1065
- DOI:
- 10.4238/vol10-2gmr1065
Genetic structure of Pilosocereus gounellei (Cactaceae) as revealed by AFLP marker to guide proposals for improvement and restoration of degraded areas in Caatinga biome
Authors: E.R. Monteiro, D.K. Strioto, A.C.S. Meirelles, C.A. Mangolin and M.F.P.S. Machado
Amplified fragment length polymorphism (AFLP) analysis was used to evaluate DNA polymorphism in Pilosocereus gounellei with the aim of differentiating samples grown in different Brazilian semiarid regions. Seven primer pairs were used to amplify 703 AFLP markers, of which 700 (99.21%) markers were polymorphic. The percentage of polymorphic markers ranged .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.2
- DOI:
- 10.4238/2015.December.15.2
Deep sequencing-based analysis of gene expression in bovine mammary epithelial cells after Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae infection
Authors: L. Xiu, Y.B. Fu, Y. Deng, X.J. Shi, Z.Y. Bian, A. Ruhan and X. Wang
The goal of this study was to characterize the transcriptome of primary bovine mammalian epithelial cells (pBMECs) and to identify candidate genes for response and resistance to Staphylococcus aureus (strain S108), Escherichia coli (strain E23), and Klebsiella pneumoniae (strain K96) infection. Using Solexa sequencing, approximately 4.9 million total sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.1
- DOI:
- 10.4238/2015.December.15.1
Cytoplasmic polymorphism and evolutionary history of plum cultivars: Insights from chloroplast DNA sequence variation of trnL-trnF spacer and aggregated trnL intron & trnL-trnF spacer
Authors: S.B. Mustapha,H. Ben Tamarzizt,G. Baraket,D. Abdallah and A. Salhi-Hannachi
We screened for polymorphisms of the non-coding region of plastid DNA in plum trees. Sequencing data from the trnL-trnF chloroplast region were used to reveal a pattern of diversity, establish phylogenetic relationships, and test the selection pressure or evolutionary demography scenario for plastome DNA. The size of the non-coding regions varied from 398.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.11
Development of novel microsatellite markers for conservation genetic studies of Vulpes vulpes (Canidae) by using next-generation sequencing method
Authors: J.-N. Yu,C.-U. Chung,K.H. Oh,B.-K. Lee and C.E. Lim
The red fox, Vulpes vulpes (Canidae), is the most widely distributed terrestrial carnivore worldwide, but this species is classified as endangered in Korea. In this study, we developed 25 polymorphic microsatellite markers that included 3-13 (mean = 6.32) alleles per locus using 22 red fox individuals. The most polymorphic locus was FR(59)TG (13 alleles) .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.12
Molecular diversity analysis of eggplant (Solanum melongena) genetic resources
Authors: Z. Ali, Z.L. Xu, D.Y. Zhang, X.L. He, S. Bahadur and J.X. Yi
Eggplant (Solanum melongena), a vegetable that is cultivated worldwide, is of considerable importance to agriculture in China. We analyzed the diversity of this plant using inter-simple sequence repeat (ISSR) and RAPD procedures to subdivide 143 Chinese-cultivated eggplants based on coefficient of parentage, genetic diversity .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1279
- DOI:
- 10.4238/vol10-2gmr1279
Effect of Fimbristylis ovata on receptor for advanced glycation end-products,proinflammatory cytokines, and cell adhesion molecule level and gene expression in U937 and bEnd.3 cell lines
Authors: S. Sukjamnong and R. Santiyanont
Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (R.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.13