Gene
Improvement of a popcorn population using selection indexes from a fourth cycle of recurrent selection program carried out in two different environments
Authors: A.T. Amaral J�ºnior, S.P. Freitas J�ºnior, R.M. Rangel, G.F. Pena, R.M. Ribeiro, R.C. Morais and A.R. Schuelter
We estimated genetic gains for popcorn varieties using selection indexes in a fourth cycle of intrapopulation recurrent selection developed in the campus of the Universidade Estadual do Norte Fluminense. Two hundred full-sib families were obtained from the popcorn population UNB-2U of the third recurrent selection cycle. The p.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr702
- DOI:
- 10.4238/vol9-1gmr702
VP6 gene diversity in Brazilian strains of porcine group C rotavirus
Authors: K.C. M�©dici, A.F. Barry, A.F. Alfieri and A.A. Alfieri
Group C rotavirus (RV-C) has been found in Brazilian pig herds; however, wild-type strains have not yet been characterized. We made a molecular analysis of a region of gene 5 in Brazilian RV-C strains. Stool samples from 11 piglets (diarrheic and with normal consistency) positive for the RV-C VP6 gene in an RT-PCR assay were s.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr715
- DOI:
- 10.4238/vol9-1gmr715
Polymorphism in the melatonin receptor gene in buffalo populations of the Brazilian Amazon
Authors: E.B. Machado, B.B. Souza, R.C. Guimar�£es, J.S.N. Azevedo, E.C. Gon�§alves, H.F.L. Ribeiro, S.T. Rolim Filho, E. Silva Filho
Buffalo farming in Brazil is increasing, as is the challenge of identifying molecular markers that will improve productivity. Therefore, the aim of this study was to analyze single nucleotide polymorphisms of the receptor gene for the hormone melatonin in buffaloes from northern Brazil by polymerase chain reactions (PCRs) and .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027960
- DOI:
- 10.4238/gmr.15027960
DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA
Authors: S. Tabasum, F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed
DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr709
- DOI:
- 10.4238/vol9-1gmr709
Genetic diversity analysis of Aspergillus flavus isolates from plants and air by ISSR markers
Authors: M.A. Mahmoud, A.M.A. El-Samawaty, M.A. Yassin and A.R.M. Abd El-Aziz
Aspergillus flavus is one of the most abundant and widely distributed fungi on earth. A. flavus produces aflatoxins (AFs), which are toxic secondary metabolites. AFs have harmful effects on public health (humans and animals) and agricultural crops. Inter-simple sequence repeat (ISSR) markers were used to analyze the genetic di.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028081
- DOI:
- 10.4238/gmr.15028081
Selection and use of reference genes in mouse mammary glands
Authors: L.Q. Han, G.Y. Yang, H.S. Zhu, Y.Y. Wang, L.F. Wang, Y.J. Guo, W.F. Lu, H.J. Li and Y.L. Wang
Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr724
- DOI:
- 10.4238/vol9-1gmr724
Genetic diversity in the germplasm of black pepper determined by EST-SSR markers
Authors: B.D. Wu, R. Fan, L.S. Hu, H.S. Wu and C.Y. Hao
This study aimed to assess genetic diversity in the germplasm of black pepper from around the world using SSR markers from EST. In total, 13 markers were selected and successfully amplified the target loci across the black pepper germplasm. All the EST-SSR markers showed high levels of polymorphisms with an average polymorphis.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15018099
- DOI:
- 10.4238/gmr.15018099
Identification of a new locus conferring antixenosis to the brown planthopper in rice cultivar Swarnalata (Oryza sativa L.)
Authors: Y.F. Qiu, L. Cheng, F. Liu and R.B. Li
The brown planthopper [Nilaparvata lugens (Stål); BPH] has caused severe damage to rice production. The identification of resistance genes and the development of BPH-resistant varieties are economical and effective ways to manage this pest. Using an F2 population from a cross between the Indica cultivars 93-11 and Swarnalata, we mapped the Qbph-8 lo.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.29.4
- DOI:
- 10.4238/2013.August.29.4
Analysis of correlations between coronary heart disease and haplotypes of the angiotensin II receptor type 1 (AGTR1) gene
Authors: L.J. Duan and X.D. Wang
This study aimed to explore correlations between haplotypes of the angiotensin II receptor type 1 (AGTR1) gene and coronary heart disease (CHD). In total, 204 patients with CHD and 206 healthy controls were genotyped using denaturing high-performance liquid chromatography between 2008 and 2014. Five polymorphic loci were found.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017457
- DOI:
- 10.4238/gmr.15017457
Isolation and characterization of novel microsatellite markers in Mercenaria mercenaria
Authors: Y.F. Ning, Z.B. Li, X.Q. Mao, B.B. Li, Y.S. Huang, Y. Yuan and J.B. Shangguan
Mercenaria mercenaria, also known as the hard clam, is widely distributed in the coastal waters of temperate and tropical areas in the Asian Pacific region. This species is widely popular in the international market, especially in the United States, Europe, and other Western countries, because of its high protein value, taste,.. Read More»
- Genet. Mol. Res. 14(1):
- gmr.15017497
- DOI:
- 10.4238/gmr.15017497