Gene
Establishment of a rabbit model of coronary artery bypass graft and endothelial nitric oxide synthase gene transfection
Authors: Y. Zhu, H.-S. Wang, X.-M. Li and Z.-W. Wang
This study established an animal model of coronary artery bypass graft (CABG) surgery. The human endothelial nitric oxide synthase (eNOS) gene was transfected into grafted arterial walls to verify transfection efficiency. Forty rabbits were randomized into the following 4 equal groups: 1) eNOS gene transfection group (eNOS group); 2) empty eNOS gene trans.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.3
- DOI:
- 10.4238/2015.February.20.3
Molecular and morphological characterization of local apple cultivars in Southern Spain
Authors: L.F. P�©rez-Romero, M.P. Su�¡rez, E. Dapena and P. Rallo
The number of local and traditional fruit cultivars in Andalusia (Southern Spain) has decreased dramatically since the 1970s when new commercial cultivars from breeding programs were introduced, replacing old varieties, and thus decreasing genetic diversity. The present study was included in a genetic resources project with the objective of identifying and p.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.4
- DOI:
- 10.4238/2015.February.20.4
A novel polymorphism of the lactoferrin gene and its association with milk composition and body traits in dairy goats
Authors: B.L. Guo, Y. Jiao, C. He, L.X. Wei, Z.H. Chang, X.P. Yue, X.Y. Lan, H. Chen and C.Z. Lei
Milk composition and body measurement traits, influenced by genes and environmental factors, play important roles in value assessments of efficiency and productivity in dairy goats. Lactoferrin (LF), involved in the efficient expression of protein in milk, is also an anabolic factor in skeletal tissue and a potent osteoblast s.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr928
- DOI:
- 10.4238/vol9-4gmr928
Identification of natural recombinants derived from PCV2a and PCV2b
Authors: J. Hu, S.L. Zhai, S.Y. Zeng, B.B. Sun, S.F. Deng, H.L. Chen, Y. Zheng,H.X. Wang, X.P. Li, J.K. Liu, S. Cheng, X. Zhou, J.Q. Zhai and M.L. Luo
Porcine circovirus type 2 (PCV2) is considered to be the main pathogen in PC-associated diseases, and significantly affects the global pig-producing industry. PCV2 continuously evolves by point mutations and genome recombinations. In the present study, we aimed to further identify recombinant PCV2 strains. We used polymerase c.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.2.12
- DOI:
- 10.4238/2015.October.2.12
Genetic diversity analysis with RAPD linked to sex identification in the sugar cane borer Diatraea saccharalis
Authors: C. Heideman, R.E.F. Munhoz, J.R. Pattaro J�ºnior and M.A. Fernandez
Diatraea saccharalis is an insect that causes considerable losses in the sugar cane crop. Our aim was to contribute to the knowledge of the biology of D. saccharalis, with the report of DNA fragments involved in the differentiation between the male and female of this species using the RAPD sex molecular marker GyakuU-13, which.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr974
- DOI:
- 10.4238/vol9-4gmr974
Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China
Authors: H.P. Xu, X.M. He, M.X. Fang, Y.S. Hu, X.Z. Jia, Q.H. Nie and X.Q. Zhang
The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou,.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.6
- DOI:
- 10.4238/2011.December.5.6
First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia
Authors: K.A. Abd-Elsalam, S. Roshdy, O.E. Amin and M. Rabani
Colletotrichum musae is the causal agent of anthracnose in banana fruits; infection by this fungal pathogen results in severe post-harvest losses. Eleven C. musae isolates were obtained from infected imported banana fruit samples with anthracnose lesions collected from different markets in Riyadh, Saudi Arabia. The pathogenic,.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr972
- DOI:
- 10.4238/vol9-4gmr972
Generation of transgenic chicks using an oviduct-specific expression system
Authors: H.A. Kaleri, S.Y. Xu and H.L. Lin
We successfully replaced the ovalbumin gene of a magnum region in chickens with a human plasminogen activator. We constructed pL-eGFP, pL-tPAGFP and pL-2.8OVtPAGFP vectors and cultured 293FT chicken embryo fibroblasts, chicken primordial germ cells, Hela C127 cells, and oviduct epithelial cells. All vectors were expressed in t.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.8.1
- DOI:
- 10.4238/2011.December.8.1
Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR
Authors: A.L. Guerra, A.V.B. Lima, F.G. Taddei and L. Castiglioni
The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We .. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr935
- DOI:
- 10.4238/vol9-4gmr935
Lack of effect of bone morphogenetic protein 2 and 4 gene polymorphisms on bone density in postmenopausal Turkish women
Authors: Z.S. ��zkan, D. Deveci, E. ��nalan Etem and H. Y�¼ce
We investigated the effect of bone morphogenetic protein 2 and 4 (BMP-2 and -4) gene polymorphisms on bone density in postmenopausal Turkish women with osteoporosis. The frequency of single-nucleotide polymorphisms (SNPs) of BMP-2 and -4 genes was analyzed in 101 osteoporotic-postmenopausal women and 52 postmenopausal women wi.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr922
- DOI:
- 10.4238/vol9-4gmr922