Freshwater
Comparative analysis of genomic DNA extraction protocols: Maxi-preparation of quality DNA for genetic evaluation and phylogenetic studies
Authors: Muhammad Naeem, Shoaib Hassan, Samra Masud, Qurban Ali, Sikandar Hayat, Ammar Danyal Naeem and Zara Naeem
Four different DNA extraction protocols were compared for ability to produce DNA from the various tissues (caudal fin, pectoral fin, pelvic fin, muscle and gills) of three different freshwater fish species (Hypophthalmichthys molitrix, Labeo rohita and Sperata sarwari). Study describes the comparison of Phenol-chloroform; salt extraction.. Read More»
- Genet. Mol. Res. 19(1):
Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin
Authors: M.P.C. Telles, R.G. Collevatti, R.S. Braga, L.B.S. Guedes, T.G. Castro, M.C. Costa, N.J. Silva-J�ºnior, R.B. Barthem and J.A.F. Diniz-Filho
Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular ge.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.8
A snapshot on prokaryotic diversity of the Solim�µes River basin (Amazon, Brazil)
Authors: D. Toyama, C.D. Santos-J�ºnior, L.T. Kishi, T.C.S. Oliveira, J.W. Garcia, H. Sarmento, F.P. Miranda and F. Henrique-Silva
The Amazon region has the largest hydrographic basin on the planet and âÂ?¨is well known for its huge biodiversity of plants and animals. However, âÂ?¨there is a lack of studies on aquatic microbial biodiversity in the âÂ?¨Solimões River, one of its main water courses. To investigate the âÂ?¨microbial biodiversi.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029567
- DOI:
- 10.4238/gmr16029567
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16
Multiple antimicrobial resistance in Enterobacteriaceae isolates from pristine freshwater
Authors: Lima-Bittencourt CI, Cursino L, Gon�§alves-Dornelas H, Pontes DS, Nardi RMD, Callisto M, Chartone-Souza E and Nascimento AMA
A freshwater enterobacterial population (N = 111) was studied for antimicrobial and mercury resistance patterns, and for its possible association with biotic and abiotic factors in that environment. Conventional biochemical tests identified Klebsiella sp, Morganella sp, Serratia sp, Escherichia sp, Enterobacter sp, Edwarsiella.. Read More»
- Genet. Mol. Res. 6(3):
Isolation and characterization of a novel acidic matrix protein hic22 from the nacreous layer of the freshwater mussel, Hyriopsis cumingii
Authors: X.J. Liu, C. Jin, L.M. Wu, S.J. Dong, S.M. Zeng and J.L. Li
Matrix proteins that either weakly acidic or unusually highly acidic have important roles in shell biomineralization. In this study, we have identified and characterized hic22, a weakly acidic matrix protein, from the nacreous layer of Hyriopsis cumingii. Total protein was extracted from the nacre using 5 M EDTA and hic22 was purified using a DEAE-sepharo.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038656
- DOI:
- 10.4238/gmr.15038656