Fish
Comparative analysis of genomic DNA extraction protocols: Maxi-preparation of quality DNA for genetic evaluation and phylogenetic studies
Authors: Muhammad Naeem, Shoaib Hassan, Samra Masud, Qurban Ali, Sikandar Hayat, Ammar Danyal Naeem and Zara Naeem
Four different DNA extraction protocols were compared for ability to produce DNA from the various tissues (caudal fin, pectoral fin, pelvic fin, muscle and gills) of three different freshwater fish species (Hypophthalmichthys molitrix, Labeo rohita and Sperata sarwari). Study describes the comparison of Phenol-chloroform; salt extraction.. Read More»
- Genet. Mol. Res. 19(1):
Prenatal diagnosis of ventricular septal defect and trisomy 7q11.23q21.3 in two fetuses: A case report
Authors: Kai Mu, Li-Sha Chen, Juan Wen, Yi Liu, Na Liu, Dong-Hua Cao
The objective of prenatal diagnosis (PD) is to provide prenatal diagnostic testing services for genetic conditions that enable families to make informed choices consistent with their individual needs and values, and to support them in deal with the outcome of such testing. This case we reported is about two fetuses with ventri.. Read More»
- Genet. Mol. Res. 17(1):
- gmr16039878
- DOI:
- 10.4238/gmr16039878
Contributions of classical and molecular cytogenetic in meiotic analysis and pollen viability for plant breeding
Authors: M.P. Lavinscky, M.M. Souza, G.S. Silva and C.A.F. Melo
The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039582
- DOI:
- 10.4238/gmr16039582
Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat
Authors: L. Cai, D.F. Sun and G.L. Sun
We studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene KN2 and bar conferring resistance to the herbicide bialaphos. The percentage of plantlets generated from induction and .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.8
Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin
Authors: M.P.C. Telles, R.G. Collevatti, R.S. Braga, L.B.S. Guedes, T.G. Castro, M.C. Costa, N.J. Silva-J�ºnior, R.B. Barthem and J.A.F. Diniz-Filho
Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular ge.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.8
Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers
Authors: I.G. Almeida, P. Ianella, M.T. Faria, S.R. Paiva and A.R. Caetano
Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.17
- DOI:
- 10.4238/2013.December.4.17
Lack of genotoxicity in Astyanax bimaculatus and Oreochromis niloticus of 17�±-methyltestosterone used in fish hatcheries to produce male monosex populations
Authors: C.L.G. Rivero-Wendt1, A.L. Miranda-Vilela1, M.F.N. Ferreira1, F.S. Amorim2, V.A.G. da Silva2, H. Louvandini3 and C.K. Grisolia1
17α-Methyltestosterone (MT) is widely used in fish hatcheries of many countries to produce male monosex populations. Its genotoxic risk to fish species is not well known and studies in other in vivo models are still inconclusive. MT was tested for genotoxicity in the fish species Oreochromis niloticus (tilapia), a target species, and Astyanax bimacu.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.13
- DOI:
- 10.4238/2013.October.24.13
Universal primers to amplify the complete mitochondrial 12S rRNA gene in marine fish species
Authors: X.X. Jin, S.L. Zhao, R.X. Wang
A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNAPhe and the 5’ region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.6
- DOI:
- 10.4238/2013.October.15.6
Chromosomal distribution of two multigene families and the unusual occurrence of an X1X1X2X2/X1X2Y sex chromosome system in the dolphinfish (Coryphaenidae): An evolutionary perspective
Authors: R.X. Soares, L.A.C. Bertollo, M.B. Cioffi, G.W.W.F. Costa and W. F. Molina
olphinfishes (Coryphaenidae) are pelagic predators distributed throughout all tropical and subtropical oceans and are very important for commercial, traditional, and sport fishing. This small family contains the Coryphaena hippurus and Coryphaena equiselis species whose chromosomal aspects remain unknown, despite recent advanc.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.19
- DOI:
- 10.4238/2014.April.3.19
Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility
Authors: H.S. An, J.W. Lee, J.Y. Park, J.I. Myeong and C.M. An
The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.2
- DOI:
- 10.4238/2013.December.9.2