Dna
RAPD and barcode analyses of groupers of the genus Epinephelus
Authors: K. Noikotr1, A. Chaveerach1, K. Pinthong2, A. Tanomtong1, R. Sudmoon1 and T. Tanee3
The diversity of Epinephelus species was investigated throughout Thailand. Random amplified polymorphic DNA successfully produced 1300 bands that were phylogenetically informative and used to construct cladograms. Values of pairwise genetic similarity (S) within species ranged from 0.65 in E. erythrurus to 0.99 in E. malabaricus. The interspecific values .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.21
- DOI:
- 10.4238/2013.November.18.21
Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility
Authors: H.S. An, J.W. Lee, J.Y. Park, J.I. Myeong and C.M. An
The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.2
- DOI:
- 10.4238/2013.December.9.2
Analysis of the genetic variability and structure of Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations from the Colombian Atlantic coast on the basis of random amplified polymorphic DNA markers
Authors: F.J. Bello, N.A. Segura and M. Ruiz-Garcia
Ochlerotatus taeniorhynchus (Diptera: Culicidae) is a mosquito, which is an efficient vector of the virus causing epidemic-epizootic Venezuelan equine encephalitis in Colombia. This study used 9 random amplified polymorphic DNA (RAPD) markers to analyze the mosquito’s genetic variability and genetic structure of 122 spec.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.6
Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China
Authors: J.X. Dai, X.M. Liu and Y.J. Wang1
The diversity of endophytic bacteria in the sand-fixation plant Caragana microphylla was investigated by amplified rDNA restriction analysis and by sequence and phylogenetic comparisons of the 16S rRNA genes. A total of 24, 19, and 17 operational taxonomic units were identified from 16S rDNA libraries of the plant roots, stems.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.7
- DOI:
- 10.4238/2014.April.3.7
A high-throughput, high-quality plant genomic DNA extraction protocol
Authors: H. Li1,2, J. Li1,2, X.H. Cong2, Y.B. Duan2,3, L. Li2, P.C. Wei2, X.Z. Lu2 and J.B. Yang2
The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block,.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.1
- DOI:
- 10.4238/2013.October.15.1
Aberrant DNA methylation of MGMT and hMLH1 genes in prediction of gastric cancer
Authors: J. Jin, L. Xie, C.H. Xie and Y.F. Zhou
We aimed to explore the association between aberrant DNA methylation of the O(6)-methylguanine-DNA methyltransferase (MGMT) and human mutL homolog 1 (hMLH1) genes with gastric cancer. A total of 283 gastric cancer patients who were confirmed by pathological diagnosis were included in our study. Aberrant DNA methylation of MGMT.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.9
Induced mitotic homologous recombination by the babesicide imidocarb dipropionate in Aspergillus nidulans diploid cells
Authors: P.A.S.R. Santos, J.R. Sant�Anna, C.C.S. Franco, L.J. Rosada, G.N.M. Esquissato and M.A.A. Castro-Prado
Imidocarb dipropionate (IMD) is a chemotherapeutic agent prescribed for the treatment and control of babesiosis; it is known to be a nucleic acid synthesis inhibitor. Although it is an effective babesicide, there are reports of persistent IMD residues retained at high levels in edible tissues of cattle, swine and sheep, raising concerns about potential ef.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.6.1
- DOI:
- 10.4238/2012.July.6.1
DNA barcoding of arid wild plants using rbcL gene sequences
Authors: S.O. Bafeel, I.A. Arif, M.A. Bakir, A.A. Al Homaidan, A.H. Al Farhan and H.A. Khan
DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.12
- DOI:
- 10.4238/2012.July.19.12
Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting
Authors: H.S. An, J.Y. Park, J.I. Myeong and C.M. An
Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and H. diversicolor diversicolor, whose morphologic.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.18
- DOI:
- 10.4238/2013.December.4.18
Range-wide phylogeography and conservation genetics of a narrowly endemic stream salamander, Pachyhynobius shangchengensis (Caudata, Hynobiidae): implications for conservation
Authors: T. Pan, H. Wang, C.-C. Hu, W.-B. Shi, K. Zhao, X. Huang and B.-W. Zhang
The Shangcheng stout salamander (Pachyhynobius shangchengensis) is an endangered amphibian endemic to the Dabie Mountains, southeast China, and is currently threatened by habitat loss and illegal poaching. Here we used the mitochondrial DNA control region sequence (768 bp) to conduct a comprehensive investigation of genetic di.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.13.9
- DOI:
- 10.4238/2014.February.13.9