Dna
Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
Authors: L. Liu, C.L. Wang, W.Y. Peng, J. Yang, M.Q. Lan, B. Zhang, J.B. Li, Y.Y. Zhu and C.Y. Li
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimeth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.1
- DOI:
- 10.4238/2015.December.28.1
Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids
Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu
Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»
- Genet. Mol. Res. 15(2):
Respiratory chain complex III deficiency in patients with tRNA-leu mutation
Authors: J. Jiang, X.L. Wang and Y.Y. Ma
The aim of this study was to investigate the clinical and genetic profiles of mitochondrial disease resulting from deficiencies in the respiratory chain complex III. Three patients, aged between 8 months and 12 years, were recruited for this study. The activities of mitochondrial respiratory chain complexes in the peripheral leucocytes were spectrophotome.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.12
- DOI:
- 10.4238/2015.December.28.12
Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin
Authors: S.O. Bafeel
The genetic diversity of Saudi locally growing sorghum (Sorghum bicolor) cultivars has not been thoroughly characterized. To understand the genomic patterns of diversification in Saudi sorghum cultivars (N = 7), random amplified polymorphic DNA (RAPD) was used as a rapid, inexpensive method for providing information regarding genomic variability below the.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.3
- DOI:
- 10.4238/2015.August.21.3
Effect of uric acid on mitochondrial function and oxidative stress in hepatocytes
Authors: Y. Yang, Y. Zhou, S. Cheng, J.L. Sun, H. Yao and L. Ma
Here, we investigated the effect of uric acid (UA) on hepatocyte mitochondria. Hepatocytes cultured in vitro were treated with varying concentrations of UA. The change in apoptotic activity was detected by flow cytometry. The DNA damage index 8-hydroxy-deoxy-guanosine (8-OHdG) and mitochondrial function indices succinate dehyd.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028644
- DOI:
- 10.4238/gmr.15028644
Molecular identification of Achyranthis Bidentatae Radix by using DNA barcoding
Authors: D.-Y. Hou, G.-P. Wang, L.-H. Zhi, H.-W. Xu, H.-L. Liang, M.-M. Yang and G.-A. Shi
Achyranthis Bidentatae Radix has a long history in China as a commonly used herb that can be used to treat various diseases, including those related to the liver, muscles, bones, and kidneys. Recently, an increase in the number of adulterants has been reported, which affects the clinical safety of Achyranthis Bidentatae Radix... Read More»
- Genet. Mol. Res. 15(2):
Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report
Authors: M.L. ChÃ?¡vez-Briones, R. HernÃ?¡ndez-CortÃ?©s, G. Jaramillo-Rangel and M. Ortega-MartÃ?Ânez
Bite mark evidence has been repeatedly found in criminal cases. Physical comparison of a bite mark to the teeth of available suspects may not always be possible. Experimental studies have shown that the analysis of DNA present in the saliva recovered from bite marks might help in the identification of individuals. However, the application of this approach.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.23
- DOI:
- 10.4238/2015.August.21.23
Effect of DNase treatment on RNA extraction from preimplantation murine embryos
Authors: J. Norhazlin, M.N.K. Nor-Ashikin, B.P. Hoh, S.H. Sheikh Abdul Kadir, S. Norita, M. Mohd-Fazirul, W.J. Wan-Hafizah, D. Razif, M.H. Rajikin and B. Abdullah
The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.1
- DOI:
- 10.4238/2015.August.28.1
Soybean Stem Fly, Melanagromyza sojae (Diptera: Agromyzidae), in the New World: detection of high genetic diversity from soybean fields in Brazil
Authors: J.A. Arnemann, W.T. Tay, T.K. Walsh H. Brier, K. Gordon,F. Hickmann, G. Ugalde and J.V.C. Guedes
Soybean Stem Fly (SSF), Melanagromyza sojae (Zehntner), belongs to the family Agromyzidae and is highly polyphagous, attacking many plant species of the family Fabaceae, including soybean and other beans. SSF is regarded as one of the most important pests in soybean fields of Asia (e.g., China, India), North East Africa (e.g.,.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028610
- DOI:
- 10.4238/gmr.15028610
Correlation between methylation of the E-Cadherin gene and malignancy of prostate cancer
Authors: S.Q. Zhang, G.Q. Zhang and L. Zhang
Prostate cancer is a common malignant tumor in males with an unclear pathogenic mechanism. As one epigenetic regulation mechanism, DNA methylation of the whole genome and specific gene(s) plays critical roles in pathogenesis, progression, diagnosis, and treatment of prostate cancer. The E-Cadherin gene is involved in cell meta.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028046
- DOI:
- 10.4238/gmr.15028046