Dna
Molecular cloning and single nucleotide polymorphism analysis of IGF2a genes in the common carp (Cyprinus carpio)
Authors: S.Y. Su, Z.J. Dong, J.Q. Qu, Z.Y. Liang, J.Q. Zhang, L.X. Ma, W. Liu, P. Xu and X.H. Yuan
We studied whether two IGF2 transcripts in common carp are similar to those found in zebrafish. The full-length IGF2a cDNA contains a 5'-terminal untranslated region (UTR) of 105 bp, a 3'-terminal UTR of 1358 bp and an open reading frame of 612 bp, which encodes a 206-amino acid protein. A 6614-bp full-length IGF2a DNA molecul.. Read More»
- Genet. Mol. Res. 11(2):
- 0.4238/2012.May.15.3
- DOI:
- 0.4238/2012.May.15.3
A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae)
Authors: J.C. Vazquez-Angulo, V. Mendez-Trujillo, D. GonzÃ?¡lez-Mendoza, A. Morales-Trejo, O. Grimaldo-Juarez and L. Cervantes-DÃ?Âaz
Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, with.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.8
- DOI:
- 10.4238/2012.May.15.8
Relationship between lymphocyte DNA fragmentation and dose of iron oxide (Fe2O3) and silicon oxide (SiO2) nanoparticles.
Authors: J. JimÃ?©nez-Villarreal, D.I. Rivas-ArmendÃ?¡riz, R.D. Arellano PÃ?©rez-Vertti, E. Olivas CalderÃ?³n, R. GarcÃ?Âa-Garza, N.D. Betancourt-MartÃ?Ânez, L.B. Serrano-Gallardo and J. MorÃ?¡n-MartÃ?Ânez
At present, the use of nanoparticles is a controversial topic, especially when analyzing their effects in human tissues. Nanoparticles (NPs) can cause oxidative stress by increasing membrane lipids peroxidation and reactive oxygen species, and decreasing intracellular glutathione. Oxidative stress plays an important role in ce.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019206
- DOI:
- 10.4238/gmr16019206
SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption
Authors: H.M.S. Canela, L.A. Takami and M.E.S. Ferreira
Invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by Aspergillus fumigatus. The available drugs are limited and this disease continues to occur at an unacceptable frequency. Gene disruption is essential in the search for new drug targets. An efficient protocol for A. fumigatus gene disrupt.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019583
- DOI:
- 10.4238/gmr16019583
Comparison of winter and summer canola (Brassica napus) genotypes in Turkey
Authors: E. Maltas H.C. Vural
We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD m.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.8.5
- DOI:
- 10.4238/2013.February.8.5
XRCC1 rs25487 polymorphism is associated with lung cancer risk in epidemiologically susceptible Chinese people
Authors: X. Wang, K.W. Ma, Y.G. Zhao, G.J. Wang, W. Li
Base excision repair (BER) plays an important role in maintaining genome integrity and anti-cancer drug resistance. Single nucleotide polymorphisms (SNPs) in BER genes were detected in 500 lung cancer patients and 500 cancer-free controls. A logistic regression model was applied to analyze the relationship between lung ca.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.3
- DOI:
- 10.4238/2015.December.1.3
Analysis of the mitochondrial COI gene and its informative potential for evolutionary inferences in the families Coreidae and Pentatomidae (Heteroptera)
Authors: H.V. Souza1, S.R.C. Marchesin2 and M.M. Itoyama1
The mitochondrial cytochrome oxidase subunit 1 (COI) gene is one of the most popular markers used for molecular systematics. Fragments of this gene are often used to infer phylogenies, particularly the region near the 5'-end, which is used by the DNA Barcoding Consortium. With a growing number of sequences being deposited in the DNA barcoding database, th.. Read More»
- Genet. Mol. Res. 15(1):
- 0.4238/gmr.15017428
- DOI:
- 0.4238/gmr.15017428
Coconut genome size determined by flow cytometry: Tall versus Dwarf types
Authors: M. Freitas Neto1, T.N.S. Pereira1, I.G.C. Geronimo1, A.O.N. Azevedo1,S.R.R. Ramos2 and M.G. Pereira1
Coconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted fro.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017470
- DOI:
- 10.4238/gmr.15017470
Genetic diversity of Burkholderia (Proteobacteria) species from the Caatinga and Atlantic rainforest biomes in Bahia, Brazil
Authors: A.C. Santini H.R.M. Santos E. Gross R.X. Corr�ªa
The genus Burkholderia (Ã?²-Proteobacteria) currently comprises more than 60 species, including parasites, symbionts and free-living organisms. Several new species of Burkholderia have recently been described showing a great diversity of phenotypes. We examined the diversity of Burkholderia spp in environmental samples collected from Caatinga and Atlantic r.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.11.13
- DOI:
- 10.4238/2013.March.11.13
Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences
Authors: B.C. Moon, W.J. Kim, Y. Ji, Y.M. Lee, Y.M. Kang and G. Choi
Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017064
- DOI:
- 10.4238/gmr.15017064