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Plant Genetics   Research Article

AtBTH1, a BTB domain-containing protein, influences sugar-responsive germination and seedling growth in Arabidopsis

Authors: J.E. Hwang, Y.J. Kim, M.H. Shin, J. Park, W. Choi, D.J. Yun and H.C. Park

Sugars function as signal molecules that regulate gene expression, growth, and development in plants, animals, and yeasts. As a contribution to understanding the molecular mechanisms of sugar responses, we identified and characterized an Arabidopsis thaliana BTB domain-containing protein of the H subfamily 1 (designated AtBTH1). The relative expression le.. Read More»

Genet. Mol. Res. 17(4):
http://dx.doi.org/gmr16039932
DOI:
http://dx.doi.org/10.4238/gmr16039932
Plant Genetics   Research Article

DNA Extraction from silica gel preserved common bean (Phaseolus vulgaris L.) leaves

Authors: Male. A.S, Kato. F, Mukankusi. C.M

Extraction of high-quality DNA from field specimen requires collection under liquid nitrogen which is not readily available in resource constrained laboratories in low- and middle-income countries (LMICs). A method of extracting DNA from silica gel preserved common bean (Phaseolus vulgaris L.) leaves is presented. Our method which does not involve the use.. Read More»

Genet. Mol. Res. 17(4):
http://dx.doi.org/gmr16039926
DOI:
http://dx.doi.org/10.4238/gmr16039926
Human Genetics   Research Article

Screening and identification of candidate genes essential for cervical lymph node metastasis in nasopharyngeal carcinoma with microarrays

Authors: Ming Fu, Yudong Ye, Chi Zhang, Zhuo Chen, Zhenchao Zhu, Yang Peng, Xiangning Zhang, Qianhui Qiu

The aim of the present study is to identify genes that are crucial for the cervical lymph node metastasis, which predicts poor prognosis of nasopharyngeal carcinoma (NPC), endemic in southern China. Microarrays with probes to determine genomic expression profile and promoter methylation status in tumor specimens were used, to .. Read More»

Genet. Mol. Res. 17(1):
gmr16039892
DOI:
10.4238/gmr16039892
Microbial Genetics   Research Article

Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species

Authors: Asaad Gaber, Hzem Hamed, Essam Elsawy

Low-cost and low-density (LCD) DNA arrays offer an easy way to detect resistance as minimal laboratory instrumentation is needed. Nucleic acid-based amplification tests allow the rapid detection of Mycobacterium tuberculosis. Recently, a real-time PCR assay for M. tuberculosis complex was introduced. Real-Time PCR and DNA-micr.. Read More»

Genet. Mol. Res. 16(4):
gmr16039837
DOI:
10.4238/gmr16039837
Plant Genetics   Research Article

Genetics Analysis of the Biggest Cashew Tree in the World

Authors: F.P.M. do Amaral, G.H. de Sá, L.A. Filgueiras, F.S. Santos Filho, C.J.R. Santos Soares, M.P.M. do Amaral, S.E. dos Santos Valente, A. N. Mendes

This study performed a genetic study of several canopies of Anacardium occidentale L. specimen called "Cashew King" (Cajueiro da Praia-PI, Brazil) in order to assess whether all canopies belong to the same plant. Leaves were collected from  different distant points, and preserved for analysis of DNA. The analysi.. Read More»

Genet. Mol. Res. 16(4):
gmr16039817
DOI:
10.4238/gmr16039817
Human Genetics   Research Article

DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer

Authors: T.D. Silva, A.V. Felipe, V.M. Vidigal, S.S. Saad, N.M. Forones

Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissu.. Read More»

Genet. Mol. Res. 16(4):
gmr16039816
DOI:
10.4238/gmr16039816
Plant Genetics   Research Article

Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers

Authors: X.Q. Fu, F.X. Yang, X.K. Lu, X.G. Wang, B.X. Yang, F.J. Liu, Y. Liu and J. Peng

Molecular identification of hybrid purity is difficult in regional trials of cotton varieties and hybrid trials. In particular, the molecular detection of hybrid purity has not yet been reported in the case of unknown parentage. In this study, we screened 5000 pairs of primers and chose 17 pairs of core simple sequence repeat .. Read More»

Genet. Mol. Res. 16(3):
gmr16039799
DOI:
10.4238/gmr16039799
Microbial Genetics   Research Article

Genetic diversity of Bacillus sp producers of amylase isolated from the soil

Authors: A.R.E.O. Xavier, E.R. Lima, A.M.E. Oliveira, L. Cardoso, J. Santos, C.H.C. Cangussu, L.N. Leite, M.C.L. Quirino, I.G.C. Júnior,D.A. Oliveira and M.A.S. Xavier

The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective.. Read More»

Genet. Mol. Res. 16(3):
gmr16039771
DOI:
10.4238/gmr16039771
Human Genetics   Research Article

Improving the PCR protocol to amplify a repetitive DNA sequence

Authors: J. Riet, L.R.V. Ramos, R.V. Lewis and L.F. Marins

Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hair.. Read More»

Genet. Mol. Res. 16(3):
gmr16039796
DOI:
10.4238/gmr16039796
Animal Genetics   Research Article

Study of genetic variability in pigs after the traditional breeding program

Authors: I.M. Ferreira, G.S. Vieira, T.F. Braga, T.C.F. Silva, M.M. Franco and R.C. Antunes

Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency .. Read More»

Genet. Mol. Res. 16(3):