Denaturation

Improving the PCR protocol to amplify a repetitive DNA sequence

Authors: J. Riet, L.R.V. Ramos, R.V. Lewis and L.F. Marins

Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hair.. Read More»

Genet. Mol. Res. 16(3):

gmr16039796

DOI:

10.4238/gmr16039796