Biplex Pcr

Internal amplification control of PCR for the Glu1-Dx5 allele in wheat

Authors: H.N. Heim, E.S.N. Vieira, L.R.T. Polo, N.K. Lima, G.J. Silva, G.A. Linde, N.B. Colauto and I. Schuster

One of the limiting factors in using dominant markers is the unique amplification of the target fragment. Therefore, failures in polymerase chain reaction (PCR) or non-amplifications can be interpreted as an absence of the allele. The possibility of false negatives implies in reduced efficiency in the selection process in gene.. Read More»

Genet. Mol. Res. 16(3):

gmr16039718

DOI:

10.4238/gmr16039718