Oncogene-induced transcriptional patterns in established cell lines as a model for in vitro analysis of tumor biology
Authors: Francisco de Paula Careta, Bruno Marcos Verbeno Azevedo,Beatriz Alves Vianna1, Greiciane Gaburro Paneto,Irineu Iester Degasperi, Marcos do Carmo Oyama,Val�©ria Fagundes, ��ngela Maria Spagnol Perrone andI�ºri Drumond Louro
Established cell lines have long been used for in vitro studies of tumor biology, enabling investigators to control growth conditions and to draw important conclusions about the oncogenic microenvironment. However, gene expression behavior in cultured cells may not always reflect the actual in vivo scenario, and analysis deriv.. Read More»
- Genet. Mol. Res. 3(3):
Less Ã?Â?mtDNA4977 than normal in various types of tumors suggests that cancer cells are essentially free of this mutation
Authors: Maria Angela C. Dani, Sergio U. Dani, Silmara P.G. Lima, Alfredo Martinez, Benedito M. Rossi, Fernando Soares, Marco A. Zago and Andrew J.G. Simpson
Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that were adjacent to t.. Read More»
- Genet. Mol. Res. 3(3):
Heritability estimates for carcass traits of cattle: a review
Authors: Angel RÃ?Âos Utrera and Lloyd Dale Van Vleck
We present estimates of heritability for carcass traits of cattle published in the scientific literature. Seventy-two papers published from 1962 to 2004, which reported estimates of heritability for carcass traits, were reviewed. The unweighted means of estimates of heritability for 14 carcass traits by slaughter end point (ag.. Read More»
- Genet. Mol. Res. 3(3):
Integrating genomics into Eucalyptus breeding
Authors: Dario Grattapaglia
The advent of high throughput genomic technologies has opened new perspectives in the speed, scale and detail with which one can investigate genes, genomes and complex traits in Eucalyptus species. A genomic approach to a more detailed understanding of important metabolic and physiological processes, which affect tree growth a.. Read More»
- Genet. Mol. Res. 3(3):
A priori choice of hybrid parents in plants
Authors: Luiz Ant�´nio dos Santos Dias, Edgard Augusto de Toledo Picoli, Rodrigo Barros Rocha and Acelino Couto Alfenas
Plant breeding deals with high-yielding genotypes. However, how best to choose parents of these genotypes remains an unsolved question. Here, we focus on a priori choice based on parental distances by means of agronomic and molecular data. Despite numerous theoretical and empirical studies, a priori choice continues to be a co.. Read More»
- Genet. Mol. Res. 3(3):
Pro domain peptide of HGCP-Iv cysteine proteinase inhibits nematode cysteine proteinases
Authors: Francine B. Silva, Jo�£o A.N. Batista, Brener M. Marra,Rodrigo R. Fragoso, Ana Carolina S. Monteiro, Edson L.Z. Figueira, and Maria F�¡tima Grossi-de-S�¡
Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propept.. Read More»
- Genet. Mol. Res. 3(3):
The potential of virus-induced gene silencing for speeding up functional characterization of plant genes
Authors: Vagner A. Benedito, Peter B. Visser, Gerco C. Angenent and Frans A. Krens
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the circumvention of plant transformation, methodological simplicity and robustness, and speedy results. These features make VIGS an attra.. Read More»
- Genet. Mol. Res. 3(3):
Macromolecular array patterns of silk gland secretion in social Hymenoptera larvae
Authors: Guilherme Gomes, Elaine C.M. Silva-Zacarin, Fernando J. Zara, Regina L.M. Silva de Moraes and Fl�¡vio H. Caetano
The cocoon, produced by most holometabolous insects, is built with silk that is usually produced by the larval salivary gland. Although this silk has been widely studied in the Lepidoptera, its composition and macromolecular arrangement remains unknown in the Hymenoptera. The macromolecular array patterns of the silk in the la.. Read More»
- Genet. Mol. Res. 3(3):
Cytogenetics of genetic counseling patients in Pelotas, Rio Grande do Sul, Brazil
Authors: A.C. Duarte1, E. Cunha, J.M. Roth, F.L.S. Ferreira, G.L. Garcias and M.G. Martino-Roth
From 1986 to 2002, we examined the chromosomal composition of 916 patients attended by two genetic counseling services in the city of Pelotas, in the Brazilian State of Rio Grande do Sul, to determine the genetic causes of their disturbances. Patterns of G-banding using trypsin and Giemsa (GTG) and C-banding using barium and G.. Read More»
- Genet. Mol. Res. 3(3):
Comparative cytogenetic studies of Curimatidae (Pisces, Characiformes) from the middle Paran�¡ River (Argentina)
Authors: M.S. Brassesco, M.C. Pastori, H.A. Roncati and A.S. Fenocchio
Almost all species of the Curimatidae family have a stable karyotype, with a diploid number of 54 metacentric (M) and submetacentric (SM) chromosomes, and one sole nucleolus organizer pair. This family has considerable specific diversity in Argentinean fluvial basins; however, no cytogenetic data are available. Eight species f.. Read More»
- Genet. Mol. Res. 3(2):