Novel and recurrent COL7A1 mutations in Chinese patients with dystrophic epidermolysis bullosa pruriginosa
Authors: K.J. Zhu, C.Y. Zhu*, Y. Zhou and Y.M. Fan
Dystrophic epidermolysis bullosa pruriginosa (DEB-Pr) is a rare subtype of dystrophic epidermolysis bullosa (DEB). This disease is characterized by severe itching, lichenoid nodules or prurigo-like lesions, and linear scarring with a predilection for the extensor limbs. Pathogenic mutations in the type VII collagen alpha 1 (CO.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.26
- DOI:
- 10.4238/2014.September.12.26
Reference gene selection for gene expression studies in lily using quantitative real-time PCR
Authors: M.F. Zhang, Q. Liu and G.X. Jia
Quantitative real-time polymerase chain reaction (qRT-PCR) is an important technology used to analyze gene-expression levels. Reference genes, which are assumed to be expressed consistently across various developmental stages and in different tissues, were selected for expression level analysis. Using digital gene expression t.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027982
- DOI:
- 10.4238/gmr.15027982
TcCYS4, a cystatin from cocoa, reduces necrosis triggered by MpNEP2 in tobacco plants
Authors: L.S. Santana, M.G.C. Costa, N.M. Pirovani, A.F. Almeida, F.C. Alvim and C.P. Pirovani
In Brazil, most cocoa bean production occurs in Southern Bahia. Witches’ broom disease arrived in this area in 1989 and has since caused heavy losses in production. The disease is caused by the basidiomycete fungus Moniliophthora perniciosa, a hemibiotrophic fungus that produces the necrosis and ethylene-inducti.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.1
- DOI:
- 10.4238/2014.September.26.1
Transcript analysis of a goat mesenteric lymph node by deep next-generation sequencing
Authors: G.X. E, Y.J. Zhao, R.S. Na and Y.F. Huang
Deep RNA sequencing (RNA-seq) provides a practical and inexpensive alternative for exploring genomic data in non-model organisms. The functional annotation of non-model mammalian genomes, such as that of goats, is still poor compared to that of humans and mice. In the current study, we performed a whole transcriptome analysis .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028262
- DOI:
- 10.4238/gmr.15028262
Identification of grapevine rootstock cultivars using expressed sequence tag-simple sequence repeats
Authors: X.C. Fan, J.Q. Chu, C.H. Liu, X. Sun and J.G. Fang
Grapevine (Vitis) rootstock varieties or cultivars are used to confer resistance and tolerance to insect and disease pests, unfavorable soil conditions, and other environmental conditions to cultivars that are susceptible to these conditions but otherwise have desired properties. The need to genotype and thoroughly id.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.2
- DOI:
- 10.4238/2014.September.26.2
Screening of inbred popcorn lines for tolerance to low phosphorus
Authors: O.J.A.P. Santos, L.S.A. Gon�§alves, C.A. Scapim, de S.M. de Sousa, C.R. Castro, V. Y. Baba and A.L.M. de Oliveira
Increasing phosphorus use efficiency in agriculture is essential for sustainable food production. Thus, the aims of this study were: i) to identify phosphorus use efficiency (PUE) in popcorn lines during the early plant stages, ii) to study the relationship between traits correlated with PUE, and iii) to analyze genetic divers.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027519
- DOI:
- 10.4238/gmr.15027519
Characterization of the alkaline/neutral invertase gene in Dendrobium officinale and its relationship with polysaccharide accumulation
Authors: F. Gao, X.F. Cao, J.P. Si, Z.Y. Chen and C.L. Duan
Dendrobium officinale is one of the most well-known traditional Chinese medicines, and polysaccharide is its main active ingredient. Many studies have investigated the synthesis and accumulation mechanisms of polysaccharide, but until recently, little was known about the molecular mechanism of how polysaccharide is synthesized.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027647
- DOI:
- 10.4238/gmr.15027647
Characterization and genetic diversity of pepper (Capsicum spp) parents and interspecific hybrids
Authors: M.P.S.D. Costa, M.M. do R�ªgo, A.P.G. da Silva, E.R. do R�ªgo and P.A. Barroso
Pepper species exhibit broad genetic diversity, which enables their use in breeding programs. The objective of this study was to characterize the diversity between the parents of different species and their interspecific hybrids using morphological and molecular markers. The parents of Capsicum annuum (UFPB-01 and -137), C. ba.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027652
- DOI:
- 10.4238/gmr.15027652
Inhibition of subcutaneously implanted human pituitary tumor cells in nude mice by LRIG1
Authors: X. Wang, X.J. He, H.Q. Xu, Z.W. Chen and H.H. Fan
The aim of this study was to explore the inhibition of subcutaneously implanted human pituitary tumor cells in nude mice by LRIG1 and its mechanism. For this study, athymic nude mice were injected with either normal pituitary tumor RC-4B/C cells or LRIG1-transfected RC-4B/C cells. We then calculated the volume inhibition rate .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027681
- DOI:
- 10.4238/gmr.15027681
Effect of weaning age on cortisol release in piglets
Authors: L.A. Li, J.J. Yang, Y. Li, L. Lv, J.J. Xie, G.M. Du, T.M. Jin, S.Y. Qin and X.L. Jiao
The effect of weaning age on the adrenal cortex, which plays a vital role in the stress response, is currently unknown. Therefore, plasma adrenocorticotropic hormone (ACTH) and cortisol levels, weights and relative weights of adrenal glands, and steroidogenesis-related protein and enzyme expression levels in piglets weaned on .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027693
- DOI:
- 10.4238/gmr.15027693