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Animal Genetics   Research Article

Enzyme activity and morphological change in the spleens of crucian carp in the Yongcheng coal mine subsidence area, China

Authors: Y.F. Yan, J.Y. Yang, and J.Y. Lin

The goal of the current study was to investigate the effects of pollution on aquatic organisms in the Yongcheng coal mine subsidence area. Crucian carp (Carassius auratus) were collected from Yongcheng natural fishpond (experimental group) and Tianmu Lake (control group), and the spleens were isolated for analysis. Subsequentl.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027782
DOI:
10.4238/gmr.15027782
Plant Genetics   Research Article

Genetic improvement of plant architecture in the common bean

Authors: V.M.P. Silva, J.A.N. Menezes J�ºnior, P.C.S. Carneiro2, J.E.S. Carneiro and C.D. Cruz

Knowledge of genetic control of plant architecture in the common bean can help breeders define the most adequate breeding strategy to optimize gains. We examined genetic control of plant architecture in the common bean by means of partial diallel crosses. Fourteen bean lines were crossed under a partial diallel scheme, in which group 1 was composed of 8 e.. Read More»

Genet. Mol. Res. 12(3):
2013.January.30.8
DOI:
10.4238/2013.January.30.8
Microbial Genetics   Research Article

Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil

Authors: G.F. Silva, V.S. Santos, N.R. Sousa, R.E. Hanada and L. Gasparotto

Black sigatoka, caused by the fungus Mycosphaerella fijiensis (anamorphic stage: Paracercospora fijiensis), was first detected in Brazil in early 1998 in the Benjamin Constant and Tabatinga municipalities in the State of Amazonas, near to where the borders of Brazil, Colombia, and Peru converge. Understanding how cultivars rea.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027797
DOI:
10.4238/gmr.15027797
Medical Genetics   Research Article

Investigating the association between XRCC1 gene polymorphisms and susceptibility to gastric cancer

Authors: S. Chen, X.C. Zhu, Y.L. Liu, C. Wang and K.G. Zhang

We carried out a hospital-based case-control study to investigate the role of XRCC1 gene Arg399Gln, Arg280His, and Arg194Trp polymorphisms in susceptibility to gastric cancer. A total of 214 gastric cancer patients and 247 control subjects were recruited between March 2013 and March 2015, and polymorphism genotype frequencies .. Read More»

Genet. Mol. Res. 15(3):
gmr.15038342
DOI:
10.4238/gmr.15038342
Animal Genetics   Research Article

RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

Authors: J.C.V. Guerra Jr., M.R.C. Issa, F.E. Carneiro, R. Strapazzon and G. Moretto

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr696
DOI:
10.4238/vol9-1gmr696
Human Genetics   Research Article

Characterization of novel polymorphic genomic microsatellite markers of Boehmeria tricuspis (Hance) Makino

Authors: Q. Tang, J.H. Chen, G.G. Zang and M.B. Luan

In the present study, 59 polymorphic microsatellite loci of Boehmeria tricuspis (Hance) Makino were developed from the specific length amplified fragment sequencing data library of genome. The number of alleles per locus ranged from two to five, and the observed and expected heterozygosities ranged from 0.0000 to 1.0000 and fr.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027882
DOI:
10.4238/gmr.15027882
Medical Genetics   Research Article

Comparative analysis of short tandem repeat data obtained by automated and gel electrophoresis techniques

Authors: U.R. Pagel, R.S. Reis, V.P. Carvalho, E.V.W. Santos, E. Zandonade, I.D. Louro and F. Paula

Short tandem repeats (STRs) are commonly used as genetic markers. The detection and analysis of STRs can be used to gather information on polymorphisms of interest to forensic geneticists. Denaturing polyacrylamide gel electrophoresis (PAGE) is an affordable method for the detection of minor sequence changes in DNA, while capi.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038436
DOI:
10.4238/gmr.15038436
Microbial Genetics   Research Article

Association of APOA1 gene polymorphisms (rs670, rs5069, and rs2070665) with dyslipidemia in the Kazakhs of Xinjiang

Authors: D.W. Feng, R.L. Ma, H. Guo, J. He, Y.Z. Yan, Muratbek, Q. Niu,S.G. Li, D.S. Rui, F. Sun, M. Zhang, J.Y. Zhang, Y.S. Ding, J.M. Liu, K. Wang and S.X. Guo

The aim of this study was to investigate the potential association between apolipoprotein A1 (APOA1) gene rs670, rs5069, and rs2070665 polymorphisms and dyslipidemia in the Kazakh population of Xinjiang, China. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) was used to identify APOA.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028094
DOI:
10.4238/gmr.15028094
Cytogenomics   Research Article

In vitro development of cloned bovine embryos produced by handmade cloning using somatic cells from distinct levels of cell culture confluence

Authors: R.P.C. Gerger, E.S. Ribeiro, F. Forell, L.R. Bertolini, J.L. Rodrigues, C.E. Ambr�³sio, M.A. Miglino, A. Mezzalira and M. Bertolini

The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bis.. Read More»

Genet. Mol. Res. 9(1):
vol9-1gmr690
DOI:
10.4238/vol9-1gmr690
Medical Genetics   Research Article

Contributions of polymorphisms in miR146a, miR196a, and miR499 to the development of hepatocellular carcinoma

Authors: L.H. Zhang, B.B. Hao, C.Y. Zhang, X.Z. Dai and F. Zhang

Hepatocellular carcinoma is one of the most common malignant tumors worldwide; it is estimated that there were 782,000 new cases in 2012. MicroRNAs (miRNAs) play an important role in carcinogenesis by regulating oncogenes and tumor suppressors. We investigated the role of miR-146a, miR-196a2, and miR-499 polymorphisms in the r.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038582
DOI:
10.4238/gmr.15038582