Improving the PCR protocol to amplify a repetitive DNA sequence
Authors: J. Riet, L.R.V. Ramos, R.V. Lewis and L.F. Marins
Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hair.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039796
- DOI:
- 10.4238/gmr16039796
Genetic diversity among 16 genotypes of Coffea arabica in the Brazilian cerrado
Authors: C.M.S. Machado, N.S. Pimentel, A. Golynsk, A. Ferreira, H.D. Vieira and F.L. Partelli
For the selection of coffee plants that have favorable characteristics, it is necessary to evaluate variables related to production. Knowledge of the genetic divergence of arabica coffee is of extreme importance, as this knowledge can be associated with plant breeding programs in order to combine genetic divergence with good p.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039794
- DOI:
- 10.4238/gmr16039794
Genetic diversity of high performance cultivars of upland and irrigated Brazilian rice.
Authors: G.R.C. Coelho, C. Brondani, L.V. Hoffmann, P.A.M.R. Valdisser, T.C.O. Borba, J.A. Mendon�§a, L.A. Rodrigues and I.P.P. de Menezes
The objective of this study was to analyze the diversity and discrimination of high-performance Brazilian rice cultivars using microsatellite markers. Twenty-nine rice cultivars belonging to EMBRAPA Arroz e Feijão germplasm bank in Brazil were genotyped by 24 SSR markers to establish their structure and genetic discrimi.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039793
- DOI:
- 10.4238/gmr16039793
Periclinal chimera technique: new plant breeding approach
Authors: P.M. Gakpetor, H. Mohammed, D. Moreti and N.M.A. Nassar
Plant interspecific periclinal chimeras are a mosaic formed by tissues from two species. They are manipulated here as an efficient plant breeding tool for cassava root yields. In this study, plants synthesized from two chimeras, designated as chimera 2 and chimera 4, were characterized morphologically and cytologically to unra.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039790
- DOI:
- 10.4238/gmr16039790
Genetic diversity of Saccharum complex using ISSR markers
Authors: L.A.R. Oliveira, C.A. Machado, M.N. Cardoso, A.C.A. Oliveira, A.L. Amaral, A.R.C. Rabbani, A.V.C. Silva and A.S. Ledo
Sugarcane (Saccharum sp, Poaceae) is native to Southeast Asia, and due to growing demand as raw material, its cultivation recently expanded to new frontiers. The genetic diversity analysis is essential for targeting strategies in the formation and maintenance of a germplasm. This study aimed to assess the genetic diversity of .. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039788
- DOI:
- 10.4238/gmr16039788
Ewes carrying the Booroola and Vacaria prolificacy alleles respond differently to ovulation induction with equine chorionic gonadotrophin
Authors: J.C.F. Moraes and C.J.H. Souza
The magnitude of ovulation rate (OR) after hormonal induction in sheep should be considered when prolific genotypes are used. We investigated for the first time the effect of the Vacaria allele and its combined effect with the Booroola prolificacy mutation on OR after hormonal treatment during breeding and anoestrous season. A.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039787
- DOI:
- 10.4238/gmr16039787
Genetic diversity of Plathymenia reticulata Benth. in fragments of Atlantic Forest in southeastern Brazil
Authors: L.C. Souza, A.L. Silva J�ºnior, M.C. Souza, S.H. Kunz and F.D. Miranda
Studies of genetic diversity in natural populations are important for the definition of conservation strategies, especially in populations reduced by processes of fragmentation and continuous forest extraction. Molecular markers stand out as interesting tools for these studies. The objective of this research was to characteriz.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039775
- DOI:
- 10.4238/gmr16039775
Genetic diversity of Schizolobium parahyba var. amazonicum (Huber ex. Ducke) Barneby, in a forest area in Brazil
Authors: A.L. Silva J�ºnior, L.C. Souza, A.G. Pereira, M.V.W. Caldeira and F.D. Miranda
Schizolobium parahyba var. amazonicum (Fabaceae) is an arboreal species, endemic to the Amazon Rainforest, popularly known as paricá. It is used on a commercial scale in the timber sector, pulp and paper production, reclamation projects in degraded and landscaped areas. However, there is no availability of genetically i.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039774
- DOI:
- 10.4238/gmr16039774
Genetic diversity in soybean genotypes using phenotypic characters and enzymatic markers
Authors: E.V. Zambiazzi, A.T. Bruzi, A.P. Sales, I.M.M. Borges, S.R. Guilherme, A.M. Zuffo, J.G. Lima, F.O. Ribeiro, A.E.S. Mendes, S.H.M Godinho and M.L.M. Carvalho
The objective of this study was to evaluate the genetic diversity of soybean cultivars by adopting phenotypic traits and enzymatic markers, the relative contribution of agronomic traits to diversity, as well as diversity between the level of technology used in soybean cultivars and genetic breeding programs in which cultivars .. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039770
- DOI:
- 10.4238/gmr16039770
Fertility restoration locus and cytoplasm types in onion
Authors: R.R. Ferreira, C.A.F. Santos and V.R. Oliveira
The objective of this study was the identification of the cytoplasmic types and the genotyping for the fertility restoration nuclear locus (Ms) in 59 onion accessions, aiming at the selection of ‘A’ and ‘B’ lines essential for the obtainment of hybrids. Three markers were used to identify the cytoplasm .. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039766
- DOI:
- 10.4238/gmr16039766