With 300 Gy of [60Co] γ-ray radiation of dry wheat seeds of Vortex 9722, the protein content, wet gluten content, sedimentation value, and hardness variation were analyzed in 341 lines in M4. Using over population mean ± 2X standard deviation as the screening standard, 8 lines with higher protein and wet gluten content and 4 lines with lower protein and wet gluten content were selected. In the M5 generation, the quality traits - silty parameters and high molecular weight glutenin subunits (HMW-GS) - were further analyzed in these 12 lines. The results showed that in the M5 generation, the quality traits in some variants were significantly different from those in the parents; the farinograms varied greatly. Eleven variants had significantly different HMW-GS bands compared to their parents. The parents had a HMW-GS composition of 5 + 14 + 15 + 12 + 9, and the variants had HMW-GS of 11 + 5 + 7 + 9 + 12 subunits or 1 + 5 + 7 + 8 + 12 subunits, indicating that the glutenin loci of these lines were mutated.