Abstract
Polymorphic microsatellite loci for Japanese Spanish mackerel (Scomberomorus niphonius)
Author(s): L. Lin, L. Zhu, S.-F. Liu, Q.-S. Tang, Y.-Q. Su and Z.-M. ZhuangWe isolated and characterized 21 polymorphic microsatellite loci in Japanese Spanish mackerel (Scomberomorus niphonius) using a (GT)13-enriched genomic library. Forty individuals were collected from Qingdao, China. We found 3 to 24 alleles per locus, with a mean of 8.8. The observed and expected heterozygosities ranged from 0.263 to 0.975 and from 0.385 to 0.946, with means of 0.655 and 0.685, respectively. Deviation from Hardy-Weinberg proportions was detected at three loci. Two loci showed evidence for null alleles. These microsatellite markers will be useful for population genetic analysis of Japanese Spanish mackerel.
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