Abstract

Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus samples obtained from farms in Gansu, China

Porcine epidemic diarrhea poses significant sanitation problems in the porcine industry, and has negatively affected the economy in recent years. In this study, 48 fecal specimens were collected from piglets from four intensive swine farms located in the Gansu Province of China. The molecular diversity and phylogenetic relationships between porcine epidemic diarrhea viruses (PEDV) prevalent in Gansu were probed, and the resultant proteins were characterized. Sequence analysis of the spike protein (S) genes showed that each specimen had unique characteristics, and that the PEDV1/S/4 strain could be differentiated from the others via a unique mutation of the S gene. The phylogeny of S glycoprotein showedthat all strains were clustered into two major groups. The four Gansu PEDVfield strains were characterized into different groups; this finding wasconsistent with the results of the protein characterization prediction. Thisanalysis additionally revealed the unique characteristics of each specimen.The results of this study could be used to elucidate the prevalence of PEDVand contribute to the prevention of PEDV in Gansu.

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