Zygotic and embryonic cotton structures were used in in vitro procedures to obtain stable plants. Zygotes (24, 48, and 72 h) and immature embryos (10 and 25 days after fertilization) were grown in MS medium supplemented with glucose, indoleacetic acid, kinetin, and activated charcoal. Regenerated seedlings were acclimatized and grown in 10-kg pots for further phenological evaluation, based on agronomic traits and possible occurrence of somaclonal effects. We found that zygotes and immature embryos at 10 days did not develop physiological competence to allow regeneration of plants, based on adopted procedures. Stable plants were achieved with embryos at 25 days, with 68% of regeneration rate. The agronomical traits of these plants were similar to those obtained via seeds. Overall, the maturation cycle of cotton plants was 120 days after acclimation. No somaclonal effect, resulting from in vitro procedures was found in regenerated plants, based on agronomical traits evaluated in normal and regenerated plants.