Abstract

Effect of flavonoid compounds extracted from Iris species in prevention of carbon tetrachloride-induced liver fibrosis in rats

We investigated the effect of flavonoid compounds extracted from species of genus Iris L. on carbon tetrachloride (CCl4)-induced rat liver fibrosis. Thirty Sprague-Dawley rats were randomly divided into normal control group, liver fibrosis model group, and drug treatment group (N = 10 each). Next, 0.2 mL/100 g CCl4 was subcutaneously injected for 6 weeks in both model and treatment rats to generate the liver fibrosis model. In the control group, an equal volume of castor oil was injected subcutaneously. Rats in the treatment group also received 100 mg·kg-1·day-1 flavonoid compounds via gastric tubes. After 6 weeks, rats were sacrificed, and their liver tissues were examined for pathological changes, including alanine aminotransferase, aspartate aminotransferase, total bilirubin, hyaluronic acid, laminin, and procollagen type-3. Liver tissues from control rats showed no significant pathological changes, while model animals showed significant liver fibrosis. In the treatment group, liver fibrosis significantly decreased compared to the model group (P < 0.05). Liver fibrotic indices, including hyaluronic acid, laminin, and procollagen type-3, in treatment rats were all significantly lower than those in the

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