All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Cloning and expression analysis of pistillata-like gene in Sedirea japonica

Author(s): Suhua Jiang, Suyan Niu, Xiuyun Yuan, Fang Liang, Shenping Xu, Mofei Wang, Bo Cui

Members of the pistillata-like (PI-like) subfamily of plant MADS-box genes play multiple roles in regulation of reproductive meristems and the specification of sepal and petal identities. However, the function of PI-like genes in the Sedirea japonica is largely unknown. In this study, a full-length of PI-like gene in Sedirea japonica (designated PI-like, GenBank No. KM975642) was cloned using 5’/3’-RACE. The PI-like gene were 921 bp length with an open reading frame (ORF) of 633 bp, which encodes a protein of 210 amino acids, with a putative molecular weight of 24.5 kDa and a theoretical isoelectric point (pI) of 9.3. It also contains a 96-bp 5′ untranslated region (UTR), and a 192-bp 3′ UTR. The result of multiple sequence alignment indicated that PI-like belongs to a Class-B MADS-box gene. In addition, the phylogenetic tree analysis revealed that PI-like gene was highly like PI-like in Phalaenopsis. The qRT-PCR results revealed that the expression of PI-like gene was higher in the tissues on florescence compared to vegetative phase, suggesting that the PI-like gene of S. japonica performed important physiological functions on florescence