Abstract

A modified acidic approach for DNA extraction from plant species containing high levels of secondary metabolites

Author(s): M.M. Cavallari, M.V.B.M. Siqueira, T.M. Val, J.C. Pavanelli, M. Monteiro, C. Grando, J.B. Pinheiro, M.I. Zucchi and M.A. Gimenes

Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This protocol does not require the use of volatile toxic reagents such as mercaptoethanol, chloroform, or phenol and allows the extraction of high-quality DNA from wild and cultivated tropical species.

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