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Medical Genetics   Research Article

Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA

Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen

In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»

Genet. Mol. Res. 14(3):